PMID- 10864096
OWN - NLM
STAT- MEDLINE
DCOM- 20000713
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 66
IP  - 24
DP  - 2000 May 5
TI  - Gastric mucosal damage induced by arecoline seizure in rats.
PG  - 2337-49
AB  - In an attempt to know the relation of seizure and gastric mucosal damage, we 
      challenged arecoline (ACL) centrally to induce seizure and investigated gastric 
      hemorrhagic injury in acid-irrigated stomachs of rats. The protective effects of 
      several drugs also were evaluated. After deprivation of food for 24 h, rats were 
      received laparotomy under diethylether-anesthesia. Both pylorus sphincters and 
      carotid esophagus were ligated. The forestomach was equipped with a cannula for 
      gastric irrigation. After recovery from anesthesia (approximately 1 h), the 
      stomach was irrigated for 2 h with an acid solution containing 100 mM HCl and 54 
      mM NaCl or the same volume of normal saline. Intracerebroventricular (i.c.v.) ACL 
      (0, 1, 3 or 10 mg/kg dissolved in 10 microl of CSF) was challenged to rats 
      immediately after gastric irrigation. The seizure in rats was produced by ACL in 
      a dose-related manner. The ulcerogenic parameters such as decrease of gastric 
      mucosal glutathione levels and increase of histamine concentrations and lipid 
      peroxide generations as well as the raise of luminal hemoglobin contents and 
      exacerbated mucosal lesions were obtained depending on the doses of ACL 
      challenged. These ulcerogenic parameters produced in ACL (10 mg/kg, i.c.v.) 
      seizure rats were markedly ameliorated by gastric vagotomy or central 
      anticholinergics. Intraperitoneal ketotifen, zinc sulfate, diphenhydramine or 
      cimetidine also produced significant (p<0.05) inhibitions of these ulcerogenic 
      parameters in ACL seizure rats. In conclusion, central ACL seizure may produce 
      gastric oxidative stress and hemorrhagic lesions via vagal nervous activation and 
      histamine release in acid-irrigated stomachs of rats.
FAU - Hung, C R
AU  - Hung CR
AD  - Department of Pharmacology, College of Medicine, National Cheng Kung University, 
      Tainan, Taiwan. crhung@mail.ncku.edu.tw
FAU - Cheng, J T
AU  - Cheng JT
FAU - Shih, C S
AU  - Shih CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Cholinergic Agonists)
RN  - 0 (Cholinergic Antagonists)
RN  - 0 (Hemoglobins)
RN  - 0 (Lipid Peroxides)
RN  - 4ALN5933BH (Arecoline)
RN  - 820484N8I3 (Histamine)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Arecoline/*pharmacology
MH  - Cholinergic Agonists/*pharmacology
MH  - Cholinergic Antagonists/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Gastric Lavage
MH  - Gastric Mucosa/*drug effects/metabolism/pathology
MH  - Gastrointestinal Contents/chemistry
MH  - Glutathione/metabolism
MH  - Hemoglobins/analysis
MH  - Histamine/metabolism
MH  - Injections, Intraventricular
MH  - Lipid Peroxides/metabolism
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Seizures/*chemically induced
MH  - Stomach Ulcer/*chemically induced/metabolism/pathology
EDAT- 2000/06/23 11:00
MHDA- 2000/07/15 11:00
CRDT- 2000/06/23 11:00
PHST- 2000/06/23 11:00 [pubmed]
PHST- 2000/07/15 11:00 [medline]
PHST- 2000/06/23 11:00 [entrez]
AID - S0024320500005646 [pii]
AID - 10.1016/s0024-3205(00)00564-6 [doi]
PST - ppublish
SO  - Life Sci. 2000 May 5;66(24):2337-49. doi: 10.1016/s0024-3205(00)00564-6.