PMID- 10864922
OWN - NLM
STAT- MEDLINE
DCOM- 20001027
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 39
DP  - 2000 Sep 29
TI  - Src homology domain 2-containing tyrosine phosphatase 2 associates with 
      intercellular adhesion molecule 1 to regulate cell survival.
PG  - 30029-36
AB  - Intercellular adhesion molecule-1 (ICAM-1) binds to the plasma protein fibrinogen 
      (Fg) to mediate leukocyte/endothelial cell interactions. In our studies, the 
      ligation of Fg to ICAM-1 on tumor necrosis factor-alpha-stimulated endothelial 
      cells resulted in the tyrosine phosphorylation of Src homology domain 2 
      (SH2)-containing phosphatase-2 (SHP-2). The ICAM-1 cytoplasmic sequence IKKYRLQ 
      conforms poorly to the concensus immunoreceptor tyrosine-based inhibition motifs 
      found in receptors that bind SHP-2. Nevertheless, the tyrosine phosphorylated 
      sequence (IKKpYRLQ) bound specifically to the SH2 domain proximal to the 
      NH(2)-terminal of SHP-2 (SHP-2-N) but not to the SH2 domain proximal on the 
      COOH-terminal side (SHP-2-C). Phosphorylated ICAM-1 bound SHP-2-N. In 
      immunoprecipitation experiments, SHP-2 associated with phosphorylated ICAM-1. 
      Cells expressing truncated ICAM-1 that lacked the cytoplasmic sequence 
      (ICAM-1(TR)) failed to associate with SHP-2. ICAM-1 containing the tyrosine to 
      alanine substitution at position 485 (ICAM-1(Y485A)) associated weakly with 
      SHP-2. Cells expressing ICAM-1(TR) and ICAM-1(Y485A) underwent apoptosis upon 
      adhesion to Fg, whereas the wild type ICAM-1 maintained cell survival. These 
      results indicate that ICAM-1 interactions with SHP-2 allow better cellular 
      survival mediated through Fg-ICAM-1 ligation.
FAU - Pluskota, E
AU  - Pluskota E
AD  - Joseph J. Jacobs Center for Thrombosis and Vascular Biology, Lerner Research 
      Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
FAU - Chen, Y
AU  - Chen Y
FAU - D'Souza, S E
AU  - D'Souza SE
LA  - eng
GR  - HL 43721/HL/NHLBI NIH HHS/United States
GR  - RR-00080/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Phosphopeptides)
RN  - 0 (Phosphoproteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 42HK56048U (Tyrosine)
RN  - 9001-32-5 (Fibrinogen)
RN  - EC 3.1.3.16 (Protein Phosphatase 2)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 11)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 6)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.1.3.48 (SH2 Domain-Containing Protein Tyrosine Phosphatases)
SB  - IM
MH  - Amino Acid Motifs
MH  - Cell Survival/*physiology
MH  - Cells, Cultured
MH  - Endothelium, Vascular/cytology/drug effects
MH  - Fibrinogen/metabolism
MH  - Intercellular Adhesion Molecule-1/*metabolism
MH  - Intracellular Signaling Peptides and Proteins
MH  - Peptide Fragments/metabolism
MH  - Phosphopeptides/metabolism
MH  - Phosphoproteins/metabolism
MH  - Phosphorylation
MH  - Protein Binding
MH  - Protein Phosphatase 2
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 11
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 6
MH  - Protein Tyrosine Phosphatases/*metabolism
MH  - Receptors, Immunologic/antagonists & inhibitors
MH  - SH2 Domain-Containing Protein Tyrosine Phosphatases
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Tyrosine/metabolism
MH  - *src Homology Domains
EDAT- 2000/06/24 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/06/24 11:00
PHST- 2000/06/24 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/06/24 11:00 [entrez]
AID - S0021-9258(18)44330-X [pii]
AID - 10.1074/jbc.M000240200 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Sep 29;275(39):30029-36. doi: 10.1074/jbc.M000240200.