PMID- 10865413 OWN - NLM STAT- MEDLINE DCOM- 20000721 LR - 20061115 IS - 0125-2208 (Print) IS - 0125-2208 (Linking) VI - 83 Suppl 1 DP - 2000 Mar TI - Identification of sex chromosome by fluorescence in situ hybridization. PG - S89-94 AB - Fluorescence in situ hybridization (FISH) is the complicated and very effective technique to determine the origin of chromosome material that cannot be identified by conventional banding techniques. Also determining the hidden sex chromosome and the percentage of mosaicism. Five peripheral blood and one cord blood sample were used to perform centromeric X and Y chromosome-specific DNA probe to determine the sex chromosome. Comparing the percentage of mosaicism between conventional cytogenetic technique and FISH technique, we found a different ratio in mosaicism. That is because the molecular cytogenetic study was the evaluation of chromosome identification in both dividing (metaphase) and non-dividing cells (interphase nuclei). FAU - Sarasophona, S AU - Sarasophona S AD - Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. FAU - Promsawat, K AU - Promsawat K FAU - Rerkamnuaychok, B AU - Rerkamnuaychok B LA - eng PT - Comparative Study PT - Journal Article PL - Thailand TA - J Med Assoc Thai JT - Journal of the Medical Association of Thailand = Chotmaihet thangphaet JID - 7507216 RN - 0 (DNA Probes) SB - IM MH - Adult MH - Child MH - DNA Probes MH - Female MH - Humans MH - *In Situ Hybridization, Fluorescence MH - Male MH - Mosaicism MH - Sampling Studies MH - Sensitivity and Specificity MH - X Chromosome/*genetics MH - Y Chromosome/*genetics EDAT- 2000/06/24 11:00 MHDA- 2000/08/01 11:00 CRDT- 2000/06/24 11:00 PHST- 2000/06/24 11:00 [pubmed] PHST- 2000/08/01 11:00 [medline] PHST- 2000/06/24 11:00 [entrez] PST - ppublish SO - J Med Assoc Thai. 2000 Mar;83 Suppl 1:S89-94.