PMID- 10865972
OWN - NLM
STAT- MEDLINE
DCOM- 20000710
LR  - 20190915
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 14
IP  - 6
DP  - 2000 Jun
TI  - Expression and responsiveness of human interleukin-18 receptor (IL-18R) on 
      hematopoietic cell lines.
PG  - 1052-9
AB  - Interleukin-18 (IL-18) is a new inflammatory cytokine sharing biological 
      functions with IL-12. The human IL-18 receptor (IL-18R) was recently identified 
      and was found to be expressed on normal peripheral blood lymphocytes. To further 
      characterize IL-18R, we analyzed IL-18R expression using a series of human 
      hematopoietic cell lines selected from various cell lineages. We found the IL-18R 
      expression on cells of T and B lineages as expected from analysis on normal 
      cells. The IL-18R expression, however, was found not to be restricted to any 
      specific maturation stages of T and B cells. In addition, we detected IL-18R 
      expression in myeloid, monocytoid, erythroid and megakaryocytic cell lines, 
      indicating that normal counterparts of these cell lineages could express IL-18R 
      and participate in in vivo reactions caused by IL-18. Biochemical studies showed 
      that IL-18R proteins exist as heterogeneous molecules ranging from 60 to 110 kDa. 
      Deglycosylation experiments indicated that the heterogeneity could not be 
      explained only by a difference in glycosylation. We also found that tumor 
      necrosis factor-alpha (TNF-alpha) modulated the IL-18R expression, which implies 
      an important in vivo effect of TNF-alpha on IL-18-induced reaction. Analyzing the 
      responsiveness of IL-18R, we found that only KG-1 responded to IL-18 stimulation. 
      This suggests that certain inhibitory mechanisms of IL-18 responsive genes are 
      involved in the all IL-18R-positive cell lines except KG-1.
FAU - Nakamura, S
AU  - Nakamura S
AD  - Fujisaki Cell Center, Hayashibara Biochemical Laboratories, Inc., Okayama, Japan.
FAU - Otani, T
AU  - Otani T
FAU - Okura, R
AU  - Okura R
FAU - Ijiri, Y
AU  - Ijiri Y
FAU - Motoda, R
AU  - Motoda R
FAU - Kurimoto, M
AU  - Kurimoto M
FAU - Orita, K
AU  - Orita K
LA  - eng
PT  - Journal Article
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (IL18R1 protein, human)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-18 Receptor alpha Subunit)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-18)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - B-Lymphocytes/immunology
MH  - Blotting, Western
MH  - Bone Marrow Cells/immunology/*metabolism
MH  - Cell Line, Transformed
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Glycosylation
MH  - Humans
MH  - Interleukin-12/pharmacology
MH  - Interleukin-18/metabolism
MH  - Interleukin-18 Receptor alpha Subunit
MH  - Receptors, Interleukin/*genetics/metabolism
MH  - Receptors, Interleukin-18
MH  - T-Lymphocytes/immunology
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Up-Regulation
EDAT- 2000/06/24 11:00
MHDA- 2000/07/15 11:00
CRDT- 2000/06/24 11:00
PHST- 2000/06/24 11:00 [pubmed]
PHST- 2000/07/15 11:00 [medline]
PHST- 2000/06/24 11:00 [entrez]
AID - 10.1038/sj.leu.2401789 [doi]
PST - ppublish
SO  - Leukemia. 2000 Jun;14(6):1052-9. doi: 10.1038/sj.leu.2401789.