PMID- 10866799
OWN - NLM
STAT- MEDLINE
DCOM- 20000816
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 267
IP  - 13
DP  - 2000 Jul
TI  - A trans-acting factor, isolated by the three-hybrid system, that influences 
      alternative splicing of the amyloid precursor protein minigene.
PG  - 4002-10
AB  - Two clones were isolated in a three-hybrid screen of a rat fetal brain P5 cDNA 
      library with an intronic splicing enhancer of the amyloid precursor protein (APP) 
      gene as RNA bait. These clones represent the rat homologues of the previously 
      described genes CUG-binding protein (CUG-BP) and Siah-binding protein (Siah-BP). 
      Both interact in a sequence-specific manner with the RNA bait used for library 
      screening as well as with the CUG repeat. In contrast, no interactions were 
      observed in the three-hybrid assay with other baits tested. In two-hybrid assays, 
      Siah-BP interacts with U2AF65 as well as with itself. EWS, an RGG-type 
      RNA-binding protein associated with Ewing sarcoma, was identified as an 
      interacting partner for the CUG-BP homologue in a two-hybrid assay for 
      protein-protein interactions performed with various factors involved in RNA 
      metabolism. Splicing assays performed by RT-PCR from cells cotransfected with 
      certain cDNAs and an APP minigene, used as a reporter, indicate exclusion of exon 
      8 if the CUG-BP homologue is present. We conclude that clone AF169013 and its 
      counterpart in human CUG-BP could be the trans-acting factors that interact with 
      the splicing enhancer downstream of exon 8, and in this way influence alternative 
      splicing of the APP minigene.
FAU - Poleev, A
AU  - Poleev A
AD  - Max-Planck-Institute for Neurobiology, Munich, Germany. andrejpoleev@yahoo.com
FAU - Hartmann, A
AU  - Hartmann A
FAU - Stamm, S
AU  - Stamm S
LA  - eng
SI  - GENBANK/AF165892
SI  - GENBANK/AF169013
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Amyloid beta-Protein Precursor/*genetics
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - *Enhancer Elements, Genetic
MH  - Humans
MH  - Molecular Sequence Data
MH  - RNA-Binding Proteins/*physiology
EDAT- 2000/06/24 11:00
MHDA- 2000/08/19 11:00
CRDT- 2000/06/24 11:00
PHST- 2000/06/24 11:00 [pubmed]
PHST- 2000/08/19 11:00 [medline]
PHST- 2000/06/24 11:00 [entrez]
AID - ejb1431 [pii]
AID - 10.1046/j.1432-1327.2000.01431.x [doi]
PST - ppublish
SO  - Eur J Biochem. 2000 Jul;267(13):4002-10. doi: 10.1046/j.1432-1327.2000.01431.x.