PMID- 10867142
OWN - NLM
STAT- MEDLINE
DCOM- 20000719
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 119
IP  - 2
DP  - 2000 Jun
TI  - Detailed genome-wide screening for inter- and intrachromosomal abnormalities by 
      sequential G-banding and RxFISH color banding of the same metaphase cells.
PG  - 94-101
AB  - While the now-classic chromosome banding methods, such as G-banding, remain the 
      techniques of choice for the initial screening for karyotypic abnormalities, 
      sometimes chromosomal rearrangements involve segments too small or too similarly 
      banded to be detected or described adequately by these techniques. The necessity 
      to use a genome-wide, fluorescence in situ hybridization (FISH)-based screening 
      technique as a complement to G-banding is especially obvious in cases where the 
      information obtained by the latter analysis does not provide an adequate guide to 
      the choice of probes for chromosome-specific FISH. Furthermore, the same 
      metaphase cells should ideally be used for both G-banding and FISH analysis to 
      overcome the scarcity of metaphases observed in many cases and to ensure the 
      correct interpretation of chromosomal aberrations in cytogenetically unstable 
      neoplasms with massive cell-to-cell karyotypic variability. We describe a 
      protocol which enables cross-species color banding (RxFISH), a new FISH-based 
      screening technique that simultaneously imparts specific color banding patterns 
      on all chromosomes, of preparations that have been G-banded and mounted for up to 
      several years, as well as a procedure allowing chromosome-specific painting of 
      the same metaphase cells to resolve whatever doubts persist after the preceding 
      G-banding and RxFISH analyses. This approach makes possible a detailed, 
      genome-wide screening for inter- and intrachromosomal abnormalities including 
      archival cases whose karyotypic rearrangements had been incompletely identified 
      by G-banding.
FAU - Teixeira, M R
AU  - Teixeira MR
AD  - Department of Genetics, Institute for Cancer Research, The Norwegian Radium 
      Hospital, Oslo, Norway.
FAU - Micci, F
AU  - Micci F
FAU - Dietrich, C U
AU  - Dietrich CU
FAU - Heim, S
AU  - Heim S
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adolescent
MH  - Aged
MH  - *Aneuploidy
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Chromosome Banding/*methods
MH  - Chromosome Painting
MH  - Chromosomes, Human/*ultrastructure
MH  - Female
MH  - *Genome
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Metaphase
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/genetics/pathology
MH  - Neoplasms/genetics/ultrastructure
MH  - Specimen Handling
EDAT- 2000/06/27 11:00
MHDA- 2000/07/25 11:00
CRDT- 2000/06/27 11:00
PHST- 2000/06/27 11:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/06/27 11:00 [entrez]
AID - S0165-4608(99)00220-4 [pii]
AID - 10.1016/s0165-4608(99)00220-4 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2000 Jun;119(2):94-101. doi: 
      10.1016/s0165-4608(99)00220-4.