PMID- 10867143
OWN - NLM
STAT- MEDLINE
DCOM- 20000719
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 119
IP  - 2
DP  - 2000 Jun
TI  - 19p deletion in recurring leiomyosarcoma lesions from the same patient.
PG  - 102-8
AB  - Ten leiomyosarcomas (LMS) affecting the same patient over a period of 3 years 
      were cytogenetically studied to detect nonrandom chromosomal changes with a 
      pathogenetic significance. All tumors, likely metastases of a previous LMS 
      presentation, were classified as small, including eight that developed before 
      chemotherapy; the diagnoses were based on standard immunohistochemistry methods 
      for smooth muscle tumors. Scoring of 613 metaphases revealed monosomy of 
      chromosome 22 in six LMS, monosomy of chromosome 19 in three, and deletion of 
      chromosome 19p in all ten. Interphase fluorescence in situ hybridization (FISH) 
      of the 22-alphoid-specific probe allowed loss of the target chromosome to be 
      detected in four tumors at higher frequencies than those detected by 
      cytogenetics. Double-color FISH of the 19p- and 19q-specific YAC performed on one 
      tumor made it possible to distinguish the monosomic and 19p deleted cells, the 
      relative frequencies of which were found to be 10% and 20%, respectively. The 
      deletion breakpoint could be mapped at 19p13 between YAC 957d12 and YAC 947g4. 
      The recurrence of the 19p deletion in a subset of tumor cells from all of the 
      analyzed LMS suggests that this structural aberration is a significant change in 
      the development of leiomyosarcomas.
FAU - Riva, P
AU  - Riva P
AD  - Department of Biology and Genetics, Medical Faculty, University of Milan, Milan, 
      Italy.
FAU - Dalpra, L
AU  - Dalpra L
FAU - Gualandri, V
AU  - Gualandri V
FAU - Volonte, M
AU  - Volonte M
FAU - Miozzo, M
AU  - Miozzo M
FAU - Malgara, R
AU  - Malgara R
FAU - Conti, A F
AU  - Conti AF
FAU - Larizza, L
AU  - Larizza L
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Adult
MH  - Biomarkers, Tumor/analysis
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 19/genetics/*ultrastructure
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - Karyotyping
MH  - Leiomyosarcoma/chemistry/*genetics/pathology
MH  - Male
MH  - Microsatellite Repeats
MH  - Neoplasm Proteins/analysis
MH  - Neoplasm Recurrence, Local
MH  - Neoplasms, Second Primary/chemistry/*genetics/pathology
MH  - Soft Tissue Neoplasms/chemistry/*genetics/pathology
EDAT- 2000/06/27 11:00
MHDA- 2000/07/25 11:00
CRDT- 2000/06/27 11:00
PHST- 2000/06/27 11:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/06/27 11:00 [entrez]
AID - S0165460899002228 [pii]
AID - 10.1016/s0165-4608(99)00222-8 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2000 Jun;119(2):102-8. doi: 
      10.1016/s0165-4608(99)00222-8.