PMID- 10869521
OWN - NLM
STAT- MEDLINE
DCOM- 20000821
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 870
IP  - 1-2
DP  - 2000 Jul 7
TI  - Early and specific expression of monocyte chemoattractant protein-1 in the 
      thalamus induced by cortical injury.
PG  - 211-21
AB  - For many years it has been known that retrograde degeneration of thalamic neurons 
      occurs following damage to the cerebral cortex, however, the molecular mechanisms 
      which control this process are unknown. Recent studies have demonstrated 
      microglial activation in thalamic nuclei well before the onset of retrograde 
      neuronal cell death. Activated monocytes and microglia synthesize factors 
      detrimental to neuronal survival as well as phagocytose damaged and dying 
      neurons. Our previous studies demonstrated that monocyte chemoattractant 
      protein-1 (MCP-1), a beta chemokine which attracts cells of monocytic origin to 
      sites of injury, is rapidly expressed in the brain following visual cortical 
      lesions. The present study examined the expression of MCP-1 messenger RNA and 
      protein in the thalamus following a visual cortical lesion. Aspiration lesions of 
      visual cortex were made in adult mice. At specific times after lesion, brains 
      were harvested and dissected into specific regions. MCP-1 message as detected 
      using northern analysis was absent in uninjured brain, but was elevated in the 
      ipsilateral thalamus as rapidly as 1 h following the lesion. In situ 
      hybridization localized MCP-1 message to subpial glial cells of the lateral 
      geniculate nucleus (LGN) of the ipsilateral thalamus after injury. ELISA showed 
      that MCP-1 protein levels were significantly elevated in the ipsilateral thalamus 
      at 6 h, peaked at 12 h, and remained above baseline levels for at least 1 week 
      post lesion. In addition, anti-GFAP staining demonstrated activated astrocytes 
      localized to the ipsilateral LGN at 24 and 72 h after injury. The early 
      expression and regional localization of MCP-1 mRNA and protein strongly suggest 
      that MCP-1 is a critical molecule in the regulation of thalamic retrograde 
      neuronal degeneration.
FAU - Muessel, M J
AU  - Muessel MJ
AD  - Department of Anatomy and Cell Biology, University of Kansas Medical Center, 3901 
      Rainbow, Kansas City, KS 66160-7400, USA.
FAU - Berman, N E
AU  - Berman NE
FAU - Klein, R M
AU  - Klein RM
LA  - eng
GR  - HD02528/HD/NICHD NIH HHS/United States
GR  - NS38282/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Chemokine CCL2)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Astrocytes/chemistry/immunology
MH  - Brain Injuries/*immunology/physiopathology
MH  - Chemokine CCL2/analysis/*genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression/immunology
MH  - Glial Fibrillary Acidic Protein/analysis
MH  - In Situ Hybridization
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Microglia/chemistry/immunology
MH  - Nerve Degeneration/immunology/physiopathology
MH  - RNA, Messenger/analysis
MH  - Thalamus/cytology/*immunology/*physiopathology
MH  - Visual Cortex/*injuries
EDAT- 2000/06/28 11:00
MHDA- 2000/08/29 11:01
CRDT- 2000/06/28 11:00
PHST- 2000/06/28 11:00 [pubmed]
PHST- 2000/08/29 11:01 [medline]
PHST- 2000/06/28 11:00 [entrez]
AID - S0006-8993(00)02450-1 [pii]
AID - 10.1016/s0006-8993(00)02450-1 [doi]
PST - ppublish
SO  - Brain Res. 2000 Jul 7;870(1-2):211-21. doi: 10.1016/s0006-8993(00)02450-1.