PMID- 10869711
OWN - NLM
STAT- MEDLINE
DCOM- 20000929
LR  - 20190910
IS  - 0165-1838 (Print)
IS  - 0165-1838 (Linking)
VI  - 81
IP  - 1-3
DP  - 2000 Jul 3
TI  - Effect of NGF, BDNF, bFGF, aFGF and cell density on NPY expression in cultured 
      rat dorsal root ganglion neurones.
PG  - 128-38
AB  - The effect of neurotrophic factors on neuropeptide Y (NPY) expression was studied 
      in adult rat dispersed dorsal root ganglion (DRG) cultures. Nerve growth factor 
      (NGF), brain-derived neurotrophic factor (BDNF), acidic fibroblast growth factor 
      (aFGF) or basic FGF was included in the culture medium during incubation for 72 
      h. In untreated cultures, around 18% of all neurones (visualized by antibodies to 
      PGP 9.5) expressed NPY-like immunoreactivity (LI). In contrast, in vivo uninjured 
      neurones do not contain detectable levels of NPY-LI. In the immunohistochemical 
      analysis aFGF increased the percentage of NPY-immunoreactive (-IR) neurones 
      1.8-fold, while NGF, BDNF or bFGF had no significant effect on NPY expression. 
      When the effect of these growth factors was monitored with non-radioactive in 
      situ hybridization, both aFGF and bFGF caused a significant increase (2.25- and 
      1.8-fold, respectively), whereas, again, NGF and BDNF had no effect. The results 
      also showed an effect of cell density on NPY expression, whereby fewer neurones 
      expressed NPY in high than in low density cultures. This difference was seen in 
      untreated as well as growth factor-treated cultures. The present results support 
      the hypothesis that DRG neurones in culture are in an axotomized state, since 
      they express NPY to about the same extent as axotomized DRG neurones in vivo. 
      Surprisingly, two growth factors of the FGF family enhance NPY expression in DRG 
      neurones, which is in apparent contrast to a published in vivo study [Ji, R.-R., 
      Zhang, Q., Pettersson, R.F., Hokfelt, T., 1996. aFGF, bFGF and NGF differentially 
      regulate neuropeptide expression in dorsal root ganglia after axotomy and induce 
      autotomy. Reg. Pept. 66, 179-189.]. Finally, NPY expression was also influenced 
      by cell density.
FAU - Kerekes, N
AU  - Kerekes N
AD  - Department of Neuroscience, Karolinska Institutet, S-171 77, Stockholm, 
      Sweden.nora.kerekes@neuro.ki.se
FAU - Landry, M
AU  - Landry M
FAU - Lundmark, K
AU  - Lundmark K
FAU - Hokfelt, T
AU  - Hokfelt T
LA  - eng
GR  - DK41301/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Auton Nerv Syst
JT  - Journal of the autonomic nervous system
JID - 8003419
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Growth Substances)
RN  - 0 (Neuropeptide Y)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Messenger)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 104781-85-3 (Fibroblast Growth Factor 1)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cells, Cultured
MH  - Fibroblast Growth Factor 1/pharmacology
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - Ganglia, Spinal/*cytology/drug effects/*metabolism
MH  - Growth Substances/*pharmacology
MH  - Image Processing, Computer-Assisted
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Male
MH  - Nerve Growth Factor/pharmacology
MH  - Neurons/*metabolism
MH  - Neuropeptide Y/*biosynthesis
MH  - Oligonucleotide Probes
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2000/06/28 11:00
MHDA- 2000/10/07 11:01
CRDT- 2000/06/28 11:00
PHST- 2000/06/28 11:00 [pubmed]
PHST- 2000/10/07 11:01 [medline]
PHST- 2000/06/28 11:00 [entrez]
AID - S0165183800001156 [pii]
AID - 10.1016/s0165-1838(00)00115-6 [doi]
PST - ppublish
SO  - J Auton Nerv Syst. 2000 Jul 3;81(1-3):128-38. doi: 10.1016/s0165-1838(00)00115-6.