PMID- 10876072
OWN - NLM
STAT- MEDLINE
DCOM- 20001010
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 288
IP  - 2
DP  - 2000 Jul 14
TI  - Nitric oxide mediated induction of cytochrome c oxidase mRNA and protein in a 
      mouse macrophage cell line.
PG  - 107-10
AB  - Neurons that express neuronal nitric oxide synthase (nNOS) are selectively spared 
      from nitric oxide (NO)-induced cytotoxicity in acute cerebral ischemia and 
      neurodegenerative conditions but the mechanism of this resistance is unknown. To 
      identify specific gene products which may mediate this resistance, we performed 
      polymerase chain reaction (PCR)-based subtractive hybridization on a mouse 
      macrophage cell line treated with either L-NG-nitroarginine methyl ester (L-NAME, 
      1 mM, 1 h), an inhibitor of NOS, or with diethylamine NONOate (DEA NONO, 200 
      microM, 1 h), an NO donor. NO-treated cultures showed an acute induction of mRNA 
      (less than 1 h after treatment) and protein (15 min) for the mitochondrial enzyme 
      cytochrome c oxidase (CcO) as shown by Northern or Western blot analysis, 
      respectively. Cytochrome c oxidase activity assay showed constant activity in 
      NO-treated cultures, as compared to L-NAME-treated cultures. NO directly inhibits 
      CcO, the terminal electron acceptor in mitochondrial oxidative respiration. 
      Up-regulation of this enzyme by NO, therefore, appears to maintain vital CcO 
      activity and cellular energy stores, thus contributing to selective sparing of 
      nNOS neurons.
FAU - Lehrer-Graiwer, J E
AU  - Lehrer-Graiwer JE
AD  - Department of Physiology, University of California at San Francisco School of 
      Medicine, 513 Parnassus Avenue, 94143-0444, San Francisco, CA, USA.
FAU - Firestein, B L
AU  - Firestein BL
FAU - Bredt, D S
AU  - Bredt DS
LA  - eng
GR  - R01-NS34822/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (RNA, Messenger)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Electron Transport Complex IV/*biosynthesis/genetics
MH  - Enzyme Induction/genetics
MH  - Macrophages/*enzymology
MH  - Mice
MH  - Nitric Oxide/*physiology
MH  - Nucleic Acid Hybridization
MH  - RNA, Messenger/*biosynthesis
MH  - Up-Regulation/genetics
EDAT- 2000/07/06 11:00
MHDA- 2000/10/14 11:01
CRDT- 2000/07/06 11:00
PHST- 2000/07/06 11:00 [pubmed]
PHST- 2000/10/14 11:01 [medline]
PHST- 2000/07/06 11:00 [entrez]
AID - S0304-3940(00)01205-2 [pii]
AID - 10.1016/s0304-3940(00)01205-2 [doi]
PST - ppublish
SO  - Neurosci Lett. 2000 Jul 14;288(2):107-10. doi: 10.1016/s0304-3940(00)01205-2.