PMID- 10876669
OWN - NLM
STAT- Publisher
LR  - 20191120
IS  - 0379-4172 (Print)
IS  - 0379-4172 (Linking)
VI  - 26
IP  - 6
DP  - 1999
TI  - [Molecular identification and marker-assisted selection of Pm21 gene conferring 
      resistance to powdery mildew in wheat] [In Process Citation].
PG  - 673-82
AB  - RAPD marker (OPH17(1400)) and SCAR markers (SCAR1400 and SCAR1265) as well as 
      fluorescence in situ hybridization (FISH) technique were used to detect wheat 
      powdery mildew resistance gene Pm21 derived from Haynaldia villosa (6AL/6VS) in 
      different genetic background of wheat breeding program. Among a total of 372 RAPD 
      amplification reactions, 28 reactions (7.53%) yield no amplification products, 
      and the amplification products of 21 reactions (5.64%) were difficult to 
      interpret the presence or absence of the OPH17(1400), indicating that RAPD marker 
      OPH17(1400) is less reproducible and reliable, so that its usage in breeding 
      program is limited. However, SCAR markers, SCAR1400 and SCAR1265, were amplified 
      in all 488 PCR reactions, suggesting that SCAR markers are highly reproducible 
      and reliable, and can be used in breeding program. The specific SCAR1265 made it 
      possible to screen a large number of genotypes reliably and rapidly in practical 
      breeding programs for the presence/absence detection between the resistant and 
      susceptible plants. After backcrossed with common wheat lines for several times, 
      no recombination between the 6VS chromosome of Haynaldia villosa and any of the 
      wheat chromosomes was detected by using FISH.
FAU - Liu, ZY
AU  - Liu ZY
AD  - Department of Plant Genetics & Breeding, China Agricultural University, Beijing.
FAU - Sun, QX
AU  - Sun QX
FAU - Li, HJ
AU  - Li HJ
FAU - Ni, ZF
AU  - Ni ZF
FAU - Yang, ZM
AU  - Yang ZM
FAU - Tang, BR
AU  - Tang BR
FAU - Yang, AD
AU  - Yang AD
FAU - Jia, X
AU  - Jia X
LA  - chi
PT  - Journal Article
PL  - China
TA  - Yi Chuan Xue Bao
JT  - Yi chuan xue bao = Acta genetica Sinica
JID - 7900784
EDAT- 2000/07/06 00:00
MHDA- 2000/07/06 00:00
CRDT- 2000/07/06 00:00
PHST- 2000/07/06 00:00 [pubmed]
PHST- 2000/07/06 00:00 [medline]
PHST- 2000/07/06 00:00 [entrez]
PST - ppublish
SO  - Yi Chuan Xue Bao. 1999;26(6):673-82.