PMID- 10877210
OWN - NLM
STAT- MEDLINE
DCOM- 20000718
LR  - 20171116
IS  - 0026-0495 (Print)
IS  - 0026-0495 (Linking)
VI  - 49
IP  - 6
DP  - 2000 Jun
TI  - Effect of triiodothyronine on reactive oxygen species generation by leukocytes, 
      indices of oxidative damage, and antioxidant reserve.
PG  - 799-803
AB  - We have examined the effect of short-term triiodothyronine (T3) administration on 
      reactive oxygen species (ROS) generation by leukocytes in 9 euthyroid subjects. 
      At a dose of 60 microg/d orally for 7 days, T3 induced a significant increase in 
      ROS generation by mononuclear cells (MNCs) from 183 +/- 102 mV at baseline to 313 
      +/- 111 mV on the seventh day (P < .02), and by polymorphonuclear leukocytes 
      (PMNLs) from 195 +/- 94 mV at baseline to 302 +/- 104 mV on the seventh day (P < 
      .02). There was also a significant increase in meta-tyrosine (P < .001) and 
      ortho-tyrosine (P < .001), known indices of oxidative damage to proteins and 
      amino acids. However, there was no increase in plasma thiobarbituric 
      acid-reactive substances (TBARS), an index of oxidative damage to lipids, and in 
      the level of carbonylated proteins, a less sensitive index to assess protein 
      oxidation. There was no decrease in the level of antioxidants such as 
      alpha-tocopherol, vitamin A, beta-carotene, lycopene, and lutein/zeaxanthin. The 
      stimulatory effect on ROS generation may reflect a generalized increase in 
      metabolic activity or may be a specific effect on NADPH oxidase in leukocyte 
      membranes. The absence of a significant change in TBARS, carbonylated proteins, 
      alpha-tocopherol, vitamin A, beta-carotene, lycopene, and lutein/zeaxanthin may 
      reflect the short duration of the increased ROS load.
FAU - Magsino, C H Jr
AU  - Magsino CH Jr
AD  - Department of Medicine, State University of New York at Buffalo, USA.
FAU - Hamouda, W
AU  - Hamouda W
FAU - Ghanim, H
AU  - Ghanim H
FAU - Browne, R
AU  - Browne R
FAU - Aljada, A
AU  - Aljada A
FAU - Dandona, P
AU  - Dandona P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Metabolism
JT  - Metabolism: clinical and experimental
JID - 0375267
RN  - 0 (Antioxidants)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 06LU7C9H1V (Triiodothyronine)
RN  - 42HK56048U (Tyrosine)
RN  - 709-16-0 (2-tyrosine)
RN  - D5YF57V4QW (3-tyrosine)
RN  - EC 1.6.3.- (NADPH Oxidases)
SB  - IM
MH  - Adult
MH  - Antioxidants/*metabolism
MH  - Female
MH  - Humans
MH  - Leukocytes/*drug effects/metabolism
MH  - Male
MH  - NADPH Oxidases/metabolism
MH  - Oxidative Stress/*drug effects
MH  - Reactive Oxygen Species/*metabolism
MH  - Thiobarbituric Acid Reactive Substances/metabolism
MH  - Thyroid Function Tests
MH  - Triiodothyronine/*pharmacology
MH  - Tyrosine/metabolism
EDAT- 2000/07/06 11:00
MHDA- 2000/07/25 11:00
CRDT- 2000/07/06 11:00
PHST- 2000/07/06 11:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/07/06 11:00 [entrez]
AID - S0026-0495(00)80070-0 [pii]
AID - 10.1053/meta.2000.6263 [doi]
PST - ppublish
SO  - Metabolism. 2000 Jun;49(6):799-803. doi: 10.1053/meta.2000.6263.