PMID- 10877758
OWN - NLM
STAT- MEDLINE
DCOM- 20000907
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 66
IP  - 7
DP  - 2000 Jul
TI  - Effect of model sorptive phases on phenanthrene biodegradation: molecular 
      analysis of enrichments and isolates suggests selection based on bioavailability.
PG  - 2703-10
AB  - Reduced bioavailability of nonpolar contaminants due to sorption to natural 
      organic matter is an important factor controlling biodegradation of pollutants in 
      the environment. We established enrichment cultures in which solid organic phases 
      were used to reduce phenanthrene bioavailability to different degrees (R. J. 
      Grosser, M. Friedrich, D. M. Ward, and W. P. Inskeep, Appl. Environ. Microbiol. 
      66:2695-2702, 2000). Bacteria enriched and isolated from contaminated soils under 
      these conditions were analyzed by denaturing gradient gel electrophoresis (DGGE) 
      and sequencing of PCR-amplified 16S ribosomal DNA segments. Compared to DGGE 
      patterns obtained with enrichment cultures containing sand or no sorptive solid 
      phase, different DGGE patterns were obtained with enrichment cultures containing 
      phenanthrene sorbed to beads of Amberlite IRC-50 (AMB), a weak cation-exchange 
      resin, and especially Biobead SM7 (SM7), a polyacrylic resin that sorbed 
      phenanthrene more strongly. SM7 enrichments selected for mycobacterial 
      phenanthrene mineralizers, whereas AMB enrichments selected for a Burkholderia 
      sp. that degrades phenanthrene. Identical mycobacterial and Burkholderia 16S rRNA 
      sequence segments were found in SM7 and AMB enrichment cultures inoculated with 
      contaminated soil from two geographically distant sites. Other closely related 
      Burkholderia sp. populations, some of which utilized phenanthrene, were detected 
      in sand and control enrichment cultures. Our results are consistent with the 
      hypothesis that different phenanthrene-utilizing bacteria inhabiting the same 
      soils may be adapted to different phenanthrene bioavailabilities.
FAU - Friedrich, M
AU  - Friedrich M
AD  - Department of Land Resources and Environmental Sciences, Montana State 
      University, Bozeman, Montana 59717, USA. friedric@mailer.uni-marburg.de
FAU - Grosser, R J
AU  - Grosser RJ
FAU - Kern, E A
AU  - Kern EA
FAU - Inskeep, W P
AU  - Inskeep WP
FAU - Ward, D M
AU  - Ward DM
LA  - eng
SI  - GENBANK/AF247476
SI  - GENBANK/AF247477
SI  - GENBANK/AF247478
SI  - GENBANK/AF247479
SI  - GENBANK/AF247480
SI  - GENBANK/AF247481
SI  - GENBANK/AF247482
SI  - GENBANK/AF247483
SI  - GENBANK/AF247484
SI  - GENBANK/AF247485
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Culture Media)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Phenanthrenes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil Pollutants)
RN  - 448J8E5BST (phenanthrene)
SB  - IM
MH  - Adsorption
MH  - Bacteria/classification/*genetics/isolation & purification/*metabolism
MH  - Biodegradation, Environmental
MH  - Biological Availability
MH  - Culture Media
MH  - DNA, Ribosomal/analysis/genetics
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Genes, rRNA
MH  - Molecular Sequence Data
MH  - Phenanthrenes/*metabolism
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - *Soil Microbiology
MH  - Soil Pollutants/*metabolism
PMC - PMC92063
OTO - NASA
OT  - Non-programmatic
EDAT- 2000/07/06 11:00
MHDA- 2000/09/09 11:01
PMCR- 2000/07/01
CRDT- 2000/07/06 11:00
PHST- 2000/07/06 11:00 [pubmed]
PHST- 2000/09/09 11:01 [medline]
PHST- 2000/07/06 11:00 [entrez]
PHST- 2000/07/01 00:00 [pmc-release]
AID - 1675 [pii]
AID - 10.1128/AEM.66.7.2703-2710.2000 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2000 Jul;66(7):2703-10. doi: 
      10.1128/AEM.66.7.2703-2710.2000.