PMID- 10877785
OWN - NLM
STAT- MEDLINE
DCOM- 20000907
LR  - 20231105
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 66
IP  - 7
DP  - 2000 Jul
TI  - Bioaugmentation of activated sludge by an indigenous 3-chloroaniline-degrading 
      Comamonas testosteroni strain, I2gfp.
PG  - 2906-13
AB  - A strain identified as Comamonas testosteroni I2 was isolated from activated 
      sludge and found to be able to mineralize 3-chloroaniline (3-CA). During the 
      mineralization, a yellow intermediate accumulated temporarily, due to the distal 
      meta-cleavage of chlorocatechol. This strain was tested for its ability to clean 
      wastewater containing 3-CA upon inoculation into activated sludge. To monitor its 
      survival, the strain was chromosomally marked with the gfp gene and designated 
      I2gfp. After inoculation into a lab-scale semicontinuous activated-sludge (SCAS) 
      system, the inoculated strain maintained itself in the sludge for at least 45 
      days and was present in the sludge flocs. After an initial adaptation period of 6 
      days, complete degradation of 3-CA was obtained during 2 weeks, while no 
      degradation at all occurred in the noninoculated control reactor. Upon further 
      operation of the SCAS system, only 50% 3-CA removal was observed. Denaturing 
      gradient gel electrophoresis (DGGE) of 16S rRNA genes revealed a dynamic change 
      in the microbial community structure of the activated sludge. The DGGE patterns 
      of the noninoculated and the inoculated reactors evolved after 7 days to 
      different clusters, which suggests an effect of strain inoculation on the 
      microbial community structure. The results indicate that bioaugmentation, even 
      with a strain originating from that ecosystem and able to effectively grow on a 
      selective substrate, is not permanent and will probably require regular 
      resupplementation.
FAU - Boon, N
AU  - Boon N
AD  - Laboratory of Microbial Ecology and Technology, Ghent University, B-9000 Ghent, 
      Belgium.
FAU - Goris, J
AU  - Goris J
FAU - De Vos, P
AU  - De Vos P
FAU - Verstraete, W
AU  - Verstraete W
FAU - Top, E M
AU  - Top EM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Aniline Compounds)
RN  - 0 (Culture Media)
RN  - 0 (Luminescent Proteins)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 6GKR52SKIY (3-chloroaniline)
RN  - SIR7XX2F1K (aniline)
SB  - IM
MH  - Aniline Compounds/*metabolism
MH  - Bacteria/genetics/isolation & purification
MH  - Biodegradation, Environmental
MH  - Bioreactors
MH  - Comamonas testosteroni/genetics/growth & development/*metabolism
MH  - Culture Media
MH  - Ecosystem
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Genes, rRNA
MH  - Green Fluorescent Proteins
MH  - Luminescent Proteins/*genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
MH  - Water Purification
PMC - PMC92090
EDAT- 2000/07/06 11:00
MHDA- 2000/09/09 11:01
PMCR- 2000/07/01
CRDT- 2000/07/06 11:00
PHST- 2000/07/06 11:00 [pubmed]
PHST- 2000/09/09 11:01 [medline]
PHST- 2000/07/06 11:00 [entrez]
PHST- 2000/07/01 00:00 [pmc-release]
AID - 1939 [pii]
AID - 10.1128/AEM.66.7.2906-2913.2000 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2000 Jul;66(7):2906-13. doi: 
      10.1128/AEM.66.7.2906-2913.2000.