PMID- 10878549
OWN - NLM
STAT- MEDLINE
DCOM- 20001121
LR  - 20061115
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 191
IP  - 3
DP  - 2000 Jul
TI  - Detection of damage to the mitochondrial genome in the oncocytic cells of 
      Warthin's tumour.
PG  - 274-81
AB  - Warthin's tumour of the salivary glands is composed of oncocytic cells containing 
      excessive numbers of mitochondria which show frequent structural abnormalities 
      and reduced metabolic function. Recent evidence of a strong association between 
      cigarette smoking and the occurrence of Warthin's tumour prompted this study, to 
      look for evidence of damage to mitochondrial DNA (mtDNA) that could be the result 
      of an increase in oxidative stress; two-colour fluorescence in situ hybridization 
      (FISH) was developed to show the distribution of mitochondria with deleted mtDNA 
      in paraffin wax-embedded material. Approximately 10% of mtDNA bears the 'common' 
      4977 bp deletion. Using the polymerase chain reaction (PCR), the 4977 bp deletion 
      was further quantified, in Warthin's tumour and age-matched normal parotid 
      control tissue. Whilst the deletion was present in all parotid tissue, its 
      presence was significantly higher in oncocytic tumour cells. In a small number of 
      controls, there was a trend towards higher concentrations of the deletion in 
      smokers.
CI  - Copyright 2000 John Wiley & Sons, Ltd.
FAU - Lewis, P D
AU  - Lewis PD
AD  - School of Biological Sciences, University of Wales Swansea, UK. 
      balewis@swansea.ac.uk
FAU - Baxter, P
AU  - Baxter P
FAU - Paul Griffiths, A
AU  - Paul Griffiths A
FAU - Parry, J M
AU  - Parry JM
FAU - Skibinski, D O
AU  - Skibinski DO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (DNA, Mitochondrial)
RN  - 0 (DNA, Neoplasm)
SB  - IM
CIN - J Pathol. 2000 Dec;192(4):561-2. PMID: 11113879
MH  - Adenolymphoma/*genetics
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - DNA, Mitochondrial/*genetics
MH  - DNA, Neoplasm/*genetics
MH  - Epithelial Cells/ultrastructure
MH  - Female
MH  - *Gene Deletion
MH  - Genome, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Parotid Neoplasms/*genetics
MH  - Polymerase Chain Reaction
MH  - Smoking/genetics
EDAT- 2000/07/06 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/07/06 11:00
PHST- 2000/07/06 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/07/06 11:00 [entrez]
AID - 10.1002/1096-9896(2000)9999:9999<::AID-PATH634>3.0.CO;2-U [pii]
AID - 10.1002/1096-9896(2000)9999:9999<::AID-PATH634>3.0.CO;2-U [doi]
PST - ppublish
SO  - J Pathol. 2000 Jul;191(3):274-81. doi: 
      10.1002/1096-9896(2000)9999:9999<::AID-PATH634>3.0.CO;2-U.