PMID- 10879731
OWN - NLM
STAT- MEDLINE
DCOM- 20000717
LR  - 20230216
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 80
IP  - 6
DP  - 2000 Jun
TI  - Proliferation-associated expression of the MEN1 gene as revealed by in situ 
      hybridization: possible role of the menin as a negative regulator of cell 
      proliferation under DNA damage.
PG  - 797-804
AB  - The gene responsible for multiple endocrine neoplasia type 1 (MEN1) has recently 
      been identified. Wide expression of the MEN1 gene in endocrine and non-endocrine 
      organs examined by northern blotting has been reported, but the detailed cellular 
      distribution of the MEN1 transcript in each tissue has not yet been examined in 
      any species. In this report, expression of the MEN1 gene in adult human tissues 
      was studied by in situ hybridization. The MEN1 transcript was widely observed in 
      all tissues examined, and an enhanced expression in relation to cell 
      proliferation was seen in some organs. Cell cycle arrest at the G1-S border 
      reduced the MEN1 mRNA level to less than 50% of that in exponentially growing 
      asynchronous cells. The expression increased as cells entered into S phase, 
      indicating cell cycle-associated transcriptional regulation of the MEN1 gene. 
      Increase or decrease of the amount of menin did not affect proliferation of CHO 
      cells under normal conditions. However, when cells were exposed to the 
      DNA-cross-linking agent, diepoxybutane, overexpression of wild-type menin 
      inhibited DNA synthesis. This effect was not observed when cells were exposed to 
      ultraviolet light. These results suggest that menin may negatively regulate cell 
      cycle under certain DNA damage.
FAU - Ikeo, Y
AU  - Ikeo Y
AD  - Department of Aging Medicine and Geriatrics, Shinshu University School of 
      Medicine, Matsumoto, Japan.
FAU - Sakurai, A
AU  - Sakurai A
FAU - Suzuki, R
AU  - Suzuki R
FAU - Zhang, M X
AU  - Zhang MX
FAU - Koizumi, S
AU  - Koizumi S
FAU - Takeuchi, Y
AU  - Takeuchi Y
FAU - Yumita, W
AU  - Yumita W
FAU - Nakayama, J
AU  - Nakayama J
FAU - Hashizume, K
AU  - Hashizume K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Adult
MH  - Animals
MH  - CHO Cells
MH  - COS Cells
MH  - Cell Cycle/*physiology
MH  - Cell Division/*physiology
MH  - Cricetinae
MH  - *DNA Damage
MH  - *Gene Expression Regulation
MH  - Genes, Tumor Suppressor
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Multiple Endocrine Neoplasia Type 1/*genetics
MH  - Neoplasm Proteins/analysis/*genetics/*metabolism
MH  - Organ Specificity
MH  - *Proto-Oncogene Proteins
MH  - Recombinant Proteins/analysis/metabolism
MH  - Transcription, Genetic
MH  - Transfection
EDAT- 2000/07/06 11:00
MHDA- 2000/07/25 11:00
CRDT- 2000/07/06 11:00
PHST- 2000/07/06 11:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/07/06 11:00 [entrez]
AID - S0023-6837(22)02143-2 [pii]
AID - 10.1038/labinvest.3780084 [doi]
PST - ppublish
SO  - Lab Invest. 2000 Jun;80(6):797-804. doi: 10.1038/labinvest.3780084.