PMID- 10879983
OWN - NLM
STAT- MEDLINE
DCOM- 20001115
LR  - 20061115
IS  - 0723-2020 (Print)
IS  - 0723-2020 (Linking)
VI  - 23
IP  - 1
DP  - 2000 Apr
TI  - Molecular evidence for genus level diversity of bacteria capable of catalyzing 
      anaerobic ammonium oxidation.
PG  - 93-106
AB  - Recently, a bacterium capable to oxidize ammonium anaerobically at a high rate 
      was identified as novel member of the Planctomycetales (Strous, M., Fuersi, J. 
      A., Kramer, E. H. M., Logemann, S., Muyzer, G., van de Pas-Schoonen, K. T., Webb, 
      R. I., Kufnen, J. G., and Jetten, M. S. M.: Nature 400, 446-449, 1999). Here we 
      investigated the microbial community structure of a trickling filter biofilm with 
      a high anaerobic ammonium oxidation activity. Fluorescence in situ hybridization 
      (FISH) with a set of nine probes designed for specific identification of the 
      recently described anaerobic ammonium oxidizer demonstrated that only one probe 
      hybridized to bacteria within the biofilm. For phylogenetic characterization of 
      putative biofilm anaerobic ammonium oxidizers a full-cycle 16S rDNA approach was 
      performed by using a Planctomycetales-specific forward primer for PCR 
      amplification. Of the twenty-five 16S rDNA fragments (1364 bp in length) 
      amplified from the biofilm, nine were affiliated to the Planctomycetales. 
      Comparative analysis showed that these sequences were more than 98.9% similar to 
      each other but only distantly related to the previously recognized anaerobic 
      ammonium oxidizer (below 91% similarity) and all other organisms represented in 
      public 16S rRNA databases (similarities of below 79%). The retrieved sequences 
      and the previously recognized anaerobic ammonium oxidizer represent two 
      well-separated groups of a deep-branching lineage within the Planctomycetales. 
      Quantitative FISH analysis with a newly designed specific probe showed that the 
      novel bacterium, provisionally classified as "Candidatus Kuenenia 
      stuttgartiensis" constituted the dominant fraction of the biofilm bacteria. In 
      situ probing revealed that ammonia-oxidizing bacteria of the beta-subclass of 
      Proteobacteria were also present, albeit in significant smaller amounts, within 
      the anoxic biofilm. Comparative sequence analysis of a stretch of the gene 
      encoding ammonia-monooxygenase (amoA) demonstrated the occurrence of the DNA of 
      at least three different populations of beta-subclass ammonia oxidizers within 
      the biofilm.
FAU - Schmid, M
AU  - Schmid M
AD  - Lehrstuhl fur Mikrobiologie, Technische Universitat Munchen, Freising, Germany.
FAU - Twachtmann, U
AU  - Twachtmann U
FAU - Klein, M
AU  - Klein M
FAU - Strous, M
AU  - Strous M
FAU - Juretschko, S
AU  - Juretschko S
FAU - Jetten, M
AU  - Jetten M
FAU - Metzger, J W
AU  - Metzger JW
FAU - Schleifer, K H
AU  - Schleifer KH
FAU - Wagner, M
AU  - Wagner M
LA  - eng
SI  - GENBANK/AF202649
SI  - GENBANK/AF202650
SI  - GENBANK/AF202651
SI  - GENBANK/AF202652
SI  - GENBANK/AF202653
SI  - GENBANK/AF202654
SI  - GENBANK/AF202655
SI  - GENBANK/AF202656
SI  - GENBANK/AF202657
SI  - GENBANK/AF202658
SI  - GENBANK/AF202659
SI  - GENBANK/AF202660
SI  - GENBANK/AF202661
SI  - GENBANK/AF202662
SI  - GENBANK/AF202663
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
SB  - IM
MH  - Anaerobiosis
MH  - Bacteria/*classification/*genetics/growth & development/metabolism
MH  - Biofilms
MH  - Bioreactors
MH  - DNA, Bacterial/analysis/genetics
MH  - DNA, Ribosomal/analysis/genetics
MH  - Filtration/instrumentation
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Nitrosomonas/genetics
MH  - Oxidation-Reduction
MH  - Oxidoreductases/genetics
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Quaternary Ammonium Compounds/*metabolism
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/microbiology
EDAT- 2000/07/06 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/07/06 11:00
PHST- 2000/07/06 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/07/06 11:00 [entrez]
AID - S0723-2020(00)80050-8 [pii]
AID - 10.1016/S0723-2020(00)80050-8 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2000 Apr;23(1):93-106. doi: 10.1016/S0723-2020(00)80050-8.