PMID- 10880694
OWN - NLM
STAT- MEDLINE
DCOM- 20000828
LR  - 20190712
IS  - 0091-3057 (Print)
IS  - 0091-3057 (Linking)
VI  - 66
IP  - 2
DP  - 2000 Jun
TI  - Factors elevating cAMP attenuate the effects of 8-OH-DPAT on lordosis behavior.
PG  - 383-8
AB  - The effects of a soluble derivative of forskolin and of two membrane-permeable 
      analogs of cAMP, dibutyryl cAMP, and 8-bromo-cAMP, on the ability of a serotonin 
      (5-HT)(1A) receptor agonist to inhibit lordosis behavior were examined. Sexually 
      receptive, proestrous rats received a bilateral infusion into the ventromedial 
      nucleus of the hypothalamus (VMN) with 68 ng of the forskolin derivative 1, 1.5, 
      2, or 2.5 h prior to infusion with 200 ng of the 5-HT(1A) receptor agonist, 
      (+/-)-8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT). Proestrous rats and 
      ovariectomized rats, hormonally primed with 25 microg estradiol benzoate and 500 
      microg progesterone, were coinfused with 200 ng 8-OH-DPAT and either 50 microg 
      dibutyryl cAMP or 5 microg 8-bromo-cAMP. In proestrous rats, prior infusion with 
      the forskolin derivative reduced the effects of the 5-HT(1A) receptor agonist on 
      lordosis behavior. The best protection occurred at 2 h; by 2.5 h after the 
      preinfusion, any protective effect had disappeared. Coinfusion with either 
      dibutyryl-cAMP or 8-bromo-cAMP reduced the effects of 8-OH-DPAT in proestrous 
      rats. In hormone-primed, ovariectomized rats, dibutyryl cAMP offered significant 
      protection against the effects of 8-OH-DPAT, but there was no protection with 
      8-bromo-cAMP. These findings are consistent with the speculation that 8-OH-DPAT's 
      inhibition of lordosis behavior results, in part, from an inhibition of adenylyl 
      cyclase, resulting from agonist activation of 5-HT(1A) receptors in the VMN. The 
      findings are also consistent with our earlier observations for differences 
      between proestrous rats and hormone-primed, ovariectomized rats in their response 
      to 5-HT receptor-active compounds.
FAU - Uphouse, L
AU  - Uphouse L
AD  - Department of Biology, Texas Woman's University, Denton, TX 76204, USA.
FAU - Maswood, S
AU  - Maswood S
FAU - Jackson, A
AU  - Jackson A
LA  - eng
GR  - 08256/PHS HHS/United States
GR  - R01 HD28419/HD/NICHD NIH HHS/United States
GR  - R01 MH51568/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Pharmacol Biochem Behav
JT  - Pharmacology, biochemistry, and behavior
JID - 0367050
RN  - 0 (Receptors, Serotonin)
RN  - 0 (Receptors, Serotonin, 5-HT1)
RN  - 0 (Serotonin Receptor Agonists)
RN  - 1F7A44V6OU (Colforsin)
RN  - 1S4CJB5ZGN (estradiol 3-benzoate)
RN  - 23583-48-4 (8-Bromo Cyclic Adenosine Monophosphate)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - 78950-78-4 (8-Hydroxy-2-(di-n-propylamino)tetralin)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - 8-Bromo Cyclic Adenosine Monophosphate/pharmacology
MH  - 8-Hydroxy-2-(di-n-propylamino)tetralin/*pharmacology
MH  - Animals
MH  - Bucladesine/pharmacology
MH  - Colforsin/pharmacology
MH  - Cyclic AMP/*metabolism
MH  - Estradiol/administration & dosage/analogs & derivatives
MH  - Female
MH  - Ovariectomy
MH  - Posture/*physiology
MH  - Proestrus/physiology
MH  - Progesterone/administration & dosage
MH  - Rats
MH  - Rats, Inbred F344
MH  - Receptors, Serotonin/drug effects
MH  - Receptors, Serotonin, 5-HT1
MH  - Serotonin Receptor Agonists/pharmacology
MH  - Sexual Behavior, Animal/*drug effects/*physiology
MH  - Ventromedial Hypothalamic Nucleus/drug effects/physiology
EDAT- 2000/07/06 11:00
MHDA- 2000/09/02 11:01
CRDT- 2000/07/06 11:00
PHST- 2000/07/06 11:00 [pubmed]
PHST- 2000/09/02 11:01 [medline]
PHST- 2000/07/06 11:00 [entrez]
AID - S0091-3057(00)00179-9 [pii]
AID - 10.1016/s0091-3057(00)00179-9 [doi]
PST - ppublish
SO  - Pharmacol Biochem Behav. 2000 Jun;66(2):383-8. doi: 
      10.1016/s0091-3057(00)00179-9.