PMID- 10881675
OWN - NLM
STAT- MEDLINE
DCOM- 20000720
LR  - 20200324
IS  - 0304-8608 (Print)
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 145
IP  - 5
DP  - 2000
TI  - Determination of the complete nucleotide sequence of a vaccine strain of porcine 
      reproductive and respiratory syndrome virus and identification of the Nsp2 gene 
      with a unique insertion.
PG  - 871-83
AB  - The complete nucleotide sequence of the genomic RNA of a vaccine strain (SP) of 
      porcine reproductive and respiratory syndrome virus (PRRSV) was determined. It 
      shares approximately 94% identity of nucleotide sequence with two recently 
      reported North American strains, 16244B and VR2332, but only 78% with a European 
      strain, Lelystad virus (LV). Its genome is the longest among the four published 
      complete sequences of PRRSV, due to an insertion in the Nsp2-encoding region. 
      Compared to Nsp2 of the North American strains and the European strain, the 
      predicted Nsp2 of strain SP contains 36 and 155 amino acid insertions, 
      respectively, near the C-terminus, in addition to several highly variable 
      regions. The insertion shows no homology with any equivalent arterivirus 
      proteins. This high sequence disparity of Nsp2 among different PRRSV isolates 
      suggested that it could be used as a marker to differentiate PRRSV genotypes. The 
      5' RACE and primer extension analysis of three North American strains 
      demonstrated that the utmost 5'-end nucleotides are conserved among PRRSV strains 
      isolated from two continents. The predicted polyprotein 1 a/b contains conserved 
      proteinase, polymerase and helicase domains responsible for polyprotein 
      processing, RNA transcription and replication.
FAU - Shen, S
AU  - Shen S
AD  - Institute of Molecular Agrobiology, National University of Singapore, Singapore.
FAU - Kwang, J
AU  - Kwang J
FAU - Liu, W
AU  - Liu W
FAU - Liu, D X
AU  - Liu DX
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - DNA, Viral/genetics
MH  - Europe
MH  - Gene Amplification
MH  - *Genes, Viral
MH  - Haplorhini
MH  - Molecular Sequence Data
MH  - North America
MH  - Open Reading Frames
MH  - Porcine respiratory and reproductive syndrome 
      virus/*genetics/immunology/isolation & purification
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Species Specificity
MH  - Swine
MH  - Viral Nonstructural Proteins/genetics
MH  - Viral Vaccines/genetics
PMC - PMC7086861
EDAT- 2000/07/06 11:00
MHDA- 2000/07/25 11:00
PMCR- 2020/03/23
CRDT- 2000/07/06 11:00
PHST- 2000/07/06 11:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/07/06 11:00 [entrez]
PHST- 2020/03/23 00:00 [pmc-release]
AID - 1450871 [pii]
AID - 10.1007/s007050050680 [doi]
PST - ppublish
SO  - Arch Virol. 2000;145(5):871-83. doi: 10.1007/s007050050680.