PMID- 10881929
OWN - NLM
STAT- MEDLINE
DCOM- 20001025
LR  - 20190905
IS  - 0885-3177 (Print)
IS  - 0885-3177 (Linking)
VI  - 21
IP  - 1
DP  - 2000 Jul
TI  - Chemokines MCP-1 and RANTES in isolated rat pancreatic acinar cells treated with 
      CCK and ethanol in vitro.
PG  - 22-31
AB  - The role of cholecystokinin (CCK) and ethanol on the expression and secretion of 
      monocyte chemoattractant protein-1 (MCP-1) and regulated on activation, normal 
      T-cell expressed and secreted (RANTES) chemokines from isolated rat pancreatic 
      acinar cells was investigated. CCK at concentrations of 1 nM and 100 nM and 
      ethanol at concentrations of 75, 200, 400, and 600 mM were used to stimulate 
      isolated acini. The levels of MCP-1 and RANTES in the incubation medium were 
      determined by enzyme-linked immunoabsorbent assay (ELISA). In the control groups, 
      MCP-1 and RANTES were secreted into the incubation medium, and both increased 
      with time. MCP-1 increased from baseline 17.6 pg/ml to 74.1 pg/ml, whereas RANTES 
      increased from 255.5 to 318.3 pg/ml at 390 min. CCK at 100 nM caused a sustained 
      increase in MCP-1 levels to 89.6 pg/ml at 390 min in the incubation medium, 
      whereas the levels of RANTES gradually decreased after 180 min and reached its 
      lowest level at 390 min. Ethanol at a concentration of 600 mM increased the 
      levels of RANTES in the incubation medium, but inhibited the levels of MCP-1 at 
      all concentrations (75, 200, 400, and 600 mM). In summary, rat pancreatic acinar 
      cells secrete MCP-1 and RANTES, and the stimulation of these chemokines by CCK 
      and ethanol suggests that they may be involved in the pathogenesis of acute 
      pancreatitis.
FAU - Yang, B M
AU  - Yang BM
AD  - Molecular Medicine Research Groups, Plymouth Postgraduate Medical School, 
      University of Plymouth, England. byang@plymouth.ac.uk
FAU - Demaine, A G
AU  - Demaine AG
FAU - Kingsnorth, A
AU  - Kingsnorth A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pancreas
JT  - Pancreas
JID - 8608542
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 3K9958V90M (Ethanol)
RN  - 9011-97-6 (Cholecystokinin)
RN  - M03GIQ7Z6P (Sincalide)
SB  - IM
MH  - Animals
MH  - Cell Survival/drug effects
MH  - Chemokine CCL2/analysis/*metabolism
MH  - Chemokine CCL5/analysis/*metabolism
MH  - Cholecystokinin/*pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Ethanol/*pharmacology
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Male
MH  - Pancreas/cytology/drug effects/*physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Sincalide/pharmacology
EDAT- 2000/07/06 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/07/06 11:00
PHST- 2000/07/06 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/07/06 11:00 [entrez]
AID - 10.1097/00006676-200007000-00048 [doi]
PST - ppublish
SO  - Pancreas. 2000 Jul;21(1):22-31. doi: 10.1097/00006676-200007000-00048.