PMID- 10882148
OWN - NLM
STAT- MEDLINE
DCOM- 20001013
LR  - 20181113
IS  - 0391-4097 (Print)
IS  - 0391-4097 (Linking)
VI  - 23
IP  - 5
DP  - 2000 May
TI  - Screening for MEN1 tumor suppressor gene mutations in sporadic pituitary tumors.
PG  - 304-9
AB  - The molecular pathogenesis of the majority of sporadic pituitary tumors is 
      largely unknown. Pituitary adenomas can develop sporadically or as a part of 
      multiple endocrine neoplasia type 1 (MEN1). The MEN1 is thought to be a tumor 
      suppressor gene based on loss of heterozygosity (LOH) for polymorphic markers on 
      11q13 in tumors of the pancreas, parathyroid, and pituitary. Most patients with 
      familial and sporadic MEN1 carry germ-line mutations in the MEN1 gene. Two 
      previous studies and recently a third one have analyzed mutations by sequencing 
      the MEN1 gene in sporadic pituitary tumors but yielded conflicting results. This 
      study was to investigate and clarify the potential role of MEN1 mutations, in 
      sporadic pituitary adenomas. First, we examined 59 sporadic pituitary adenomas by 
      analyzing LOH on 11q13 in the MEN1 minimal interval with microsatellite analysis. 
      We found 3 tumors with LOH in 1 to 4 polymorphic markers in the MEN1 region. 
      Sequencing analysis did not reveal any mutations in the coding region of the MEN1 
      gene. However, we found 3 polymorphisms, one of which was a novel CAC to CAT 
      transition encoding His433His, in exon 9. The data show that while LOH occurs in 
      some sporadic pituitary tumors, inactivating mutations of the tumor suppressor 
      gene MEN1 are rare. These results also suggest there may be another additional 
      tumor suppressor gene at this locus which is involved in the pathogenesis of 
      sporadic pituitary neoplasms.
FAU - Evans, C O
AU  - Evans CO
AD  - Department of Neurosurgery and Laboratory of Molecular Neurosurgery and 
      Biotechnology, Emory University School of Medicine, Atlanta, GA 30322, USA.
FAU - Brown, M R
AU  - Brown MR
FAU - Parks, J S
AU  - Parks JS
FAU - Oyesiku, N M
AU  - Oyesiku NM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Italy
TA  - J Endocrinol Invest
JT  - Journal of endocrinological investigation
JID - 7806594
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adenoma/*genetics
MH  - Adult
MH  - Aged
MH  - Autoradiography
MH  - Base Sequence
MH  - Chromosomes, Human, Pair 11
MH  - DNA/chemistry
MH  - Female
MH  - *Genes, Tumor Suppressor
MH  - Humans
MH  - Loss of Heterozygosity
MH  - Male
MH  - Middle Aged
MH  - Multiple Endocrine Neoplasia Type 1/*genetics
MH  - *Mutation
MH  - Pituitary Neoplasms/*genetics
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Genetic
MH  - Sequence Analysis, DNA
EDAT- 2000/07/06 11:00
MHDA- 2000/10/21 11:01
CRDT- 2000/07/06 11:00
PHST- 2000/07/06 11:00 [pubmed]
PHST- 2000/10/21 11:01 [medline]
PHST- 2000/07/06 11:00 [entrez]
AID - 10.1007/BF03343727 [doi]
PST - ppublish
SO  - J Endocrinol Invest. 2000 May;23(5):304-9. doi: 10.1007/BF03343727.