PMID- 10882672
OWN - NLM
STAT- MEDLINE
DCOM- 20000912
LR  - 20240315
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 7
IP  - 4
DP  - 2000 Jul
TI  - Effects of androgen treatment on expression of macrophage Fcgamma receptors.
PG  - 682-6
AB  - Macrophage Fcgamma receptors (FcgammaRs) play an important role in the host 
      defense against infection and in the pathophysiology of immune cytopenias. 
      Modulation of macrophage FcgammaR expression is a potential therapeutic approach 
      to immune disorders. Glucocorticoids and progesterones decrease macrophage 
      FcgammaR expression. We assessed the effect of treatment with androgens and 
      antiandrogens on the expression of macrophage FcgammaRs using an experimental 
      guinea pig model. Four androgens (testosterone, dihydrotestosterone, mesterolone, 
      and danazol) and five antiandrogens (flutamide, nilutamide, cyproterone acetate, 
      spironolactone, and finasteride) were studied. Following in vivo treatment of 
      guinea pigs, we determined the clearance of immunoglobulin G (IgG)-sensitized 
      erythrocytes in vivo, the binding of IgG-sensitized erythrocytes by isolated 
      splenic macrophages, and splenic macrophage FcgammaR cell surface expression. All 
      of the androgens impaired the clearance of IgG-sensitized erythrocytes by 
      decreasing splenic macrophage FcgammaR expression. Dihydrotestosterone and 
      mesterolone were more effective than testosterone or dihydrotestosterone. Flow 
      cytometry and fluorescence microscopy with monoclonal antibodies demonstrated 
      that the androgens decreased the cell surface expression of FcgammaR1,2 more than 
      that of FcgammaR2. Antiandrogens did not significantly alter macrophage FcgammaR 
      expression. Nevertheless, antiandrogens counteracted the effects of androgens on 
      macrophage FcgammaR expression. These data indicate that androgens impair the 
      clearance of IgG-coated cells by decreasing splenic macrophage FcgammaR 
      expression. Thus, androgens other than danazol are candidate drugs for the 
      treatment of immune disorders.
FAU - Gomez, F
AU  - Gomez F
AD  - Hospital Universitario de Puerto Real/S.A.S., Department of Medicine, School of 
      Medicine, University of Cadiz, Cadiz, Spain. fgomez@telprof.es
FAU - Ruiz, P
AU  - Ruiz P
FAU - Lopez, R
AU  - Lopez R
FAU - Rivera, C
AU  - Rivera C
FAU - Romero, S
AU  - Romero S
FAU - Bernal, J A
AU  - Bernal JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (Androgens)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, IgG)
SB  - IM
MH  - Androgens/*pharmacology
MH  - Animals
MH  - Cells, Cultured
MH  - Guinea Pigs
MH  - Immunoglobulin G/immunology
MH  - Macrophages/*immunology
MH  - Male
MH  - Receptors, IgG/*biosynthesis/immunology
PMC - PMC95934
EDAT- 2000/07/07 11:00
MHDA- 2000/09/19 11:01
PMCR- 2000/07/01
CRDT- 2000/07/07 11:00
PHST- 2000/07/07 11:00 [pubmed]
PHST- 2000/09/19 11:01 [medline]
PHST- 2000/07/07 11:00 [entrez]
PHST- 2000/07/01 00:00 [pmc-release]
AID - 0260 [pii]
AID - 10.1128/CDLI.7.4.682-686.2000 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2000 Jul;7(4):682-6. doi: 10.1128/CDLI.7.4.682-686.2000.