PMID- 10882777
OWN - NLM
STAT- MEDLINE
DCOM- 20000929
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 871
IP  - 1
DP  - 2000 Jul 14
TI  - A subset of periglomerular neurons in the rat accessory olfactory bulb may be 
      excited by GABA through a Na(+)-dependent mechanism.
PG  - 7-15
AB  - The periglomerular (PG) cells of the accessory olfactory bulb (AOB) are GABAergic 
      interneurons which receive input from the vomeronasal sensory neurons and form 
      dendrodendritic synapses with each other and with mitral cells. Their 
      electrophysiological properties have not been investigated. We have developed a 
      novel method of isolating PG cells from the AOB, and used the whole-cell patch 
      and gramicidin-perforated patch clamp techniques to measure their basic 
      electrophysiological characteristics and their response to GABA. PG cells were 
      found to be excitable neurons with voltage-gated Na(+) and K(+) currents, though 
      it was very difficult to get PG cells to fire an action potential. The 
      voltage-gated Na(+) currents of PG cells activate at more positive potentials 
      than those of typical CNS neurons. PG cells respond to GABA with currents in 
      which GABA(A) receptors play a significant role. A subset ( approximately 40%) of 
      PG cells respond to GABA with currents which have unusually high reversal 
      potentials, indicating that GABA may be excitatory to these neurons. This 
      phenomenon cannot be explained entirely by elevation of intracellular chloride 
      concentrations, and is dependent on the presence of extracellular sodium.
FAU - Goldmakher, G V
AU  - Goldmakher GV
AD  - Department of Physiology, UT Southwestern Medical Center, 75235, Dallas, TX, USA. 
      goldmakh2utsw.swmed.edu
FAU - Moss, R L
AU  - Moss RL
LA  - eng
GR  - MH41784/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Potassium Channel Blockers)
RN  - 0 (Potassium Channels)
RN  - 0 (Receptors, GABA-A)
RN  - 0 (Sodium Channel Blockers)
RN  - 0 (Sodium Channels)
RN  - 1405-97-6 (Gramicidin)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - 66-40-0 (Tetraethylammonium)
RN  - 9NEZ333N27 (Sodium)
SB  - IM
MH  - Action Potentials/drug effects
MH  - Animals
MH  - Cell Separation
MH  - Female
MH  - Gramicidin
MH  - Neurons/*cytology/drug effects/*physiology
MH  - Olfactory Bulb/*cytology/physiology
MH  - Patch-Clamp Techniques
MH  - Potassium Channel Blockers
MH  - Potassium Channels/physiology
MH  - Rats
MH  - Receptors, GABA-A/physiology
MH  - Sodium/*physiology
MH  - Sodium Channel Blockers
MH  - Sodium Channels/physiology
MH  - Tetraethylammonium/pharmacology
MH  - Tetrodotoxin/pharmacology
MH  - gamma-Aminobutyric Acid/*pharmacology
EDAT- 2000/07/07 11:00
MHDA- 2000/10/07 11:01
CRDT- 2000/07/07 11:00
PHST- 2000/07/07 11:00 [pubmed]
PHST- 2000/10/07 11:01 [medline]
PHST- 2000/07/07 11:00 [entrez]
AID - S0006-8993(00)02282-4 [pii]
AID - 10.1016/s0006-8993(00)02282-4 [doi]
PST - ppublish
SO  - Brain Res. 2000 Jul 14;871(1):7-15. doi: 10.1016/s0006-8993(00)02282-4.