PMID- 10882791 OWN - NLM STAT- MEDLINE DCOM- 20000929 LR - 20190614 IS - 0006-8993 (Print) IS - 0006-8993 (Linking) VI - 871 IP - 1 DP - 2000 Jul 14 TI - Nerve growth factor and neurotrophin-3 modulate the rabies infection of adult sensory neurons in primary cultures. PG - 120-6 AB - With the aim of determining if the proportion of rabies virus (RV)-infected adult neurons from dorsal root ganglion are affected by in vitro treatment with different neurotrophins, experiments using Nerve Growth Factor (NGF), Brain Derived Neurotrophic Factor (BDNF) or Neurotrophin-3 (NT-3) as supplements for cells in culture were performed. Cultures treated with three different concentrations of each of the neurotrophins mentioned were infected with Challenge Virus Standard RV strain. An indirect immunoperoxidase technique was performed for the detection and counting of infected cells. NGF (2 ngml(-1) and 10 ngml(-1)) and NT-3 (1 ngml(-1) and 5 ngml(-1)) induced a significant reduction of infected neurons. None of the cultures treated with BDNF showed changes in the percentage of infected neurons. Likewise, the proportion of infected non-neuronal cells (Schwann cells and fibroblasts) was not altered by the treatment with neurotrophins. In addition, morphometric analysis of total and virus-immunoreactive neurons in culture were carried out, the neurotrophin treatment induced variations in the profile of neurons preferentially infected, since cell diameters in the infected cell population are different in the presence of NGF and NT-3. Data presented here could indicate a putative participation of neurotrophin receptor or biochemical modifications induced by neurotrophin treatment that affect the infection. The primary culture of dorsal root ganglion cells from adult mice is a very useful model for studying the basic phenomena of the RV-neuron interaction. FAU - Castellanos, J E AU - Castellanos JE AD - Laboratorio de Neurociencias, Instituto Nacional de Salud, Av. Eldorado, Carrera 50, Santafe de Bogota, Colombia. jcastellanos@hemagogus.ins.gov.co FAU - Martinez, M AU - Martinez M FAU - Acosta, O AU - Acosta O FAU - Hurtado, H AU - Hurtado H LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Brain Res JT - Brain research JID - 0045503 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Neurotrophin 3) RN - 9061-61-4 (Nerve Growth Factor) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/pharmacology MH - Cell Count MH - Cells, Cultured MH - Dose-Response Relationship, Drug MH - Fibroblasts/drug effects/virology MH - Ganglia, Spinal/cytology/drug effects/*virology MH - Mice MH - Nerve Growth Factor/*pharmacology MH - Neurons, Afferent/cytology/drug effects/*virology MH - Neurotrophin 3/*pharmacology MH - Rabies virus/drug effects/*physiology MH - Schwann Cells/drug effects/virology EDAT- 2000/07/07 11:00 MHDA- 2000/10/07 11:01 CRDT- 2000/07/07 11:00 PHST- 2000/07/07 11:00 [pubmed] PHST- 2000/10/07 11:01 [medline] PHST- 2000/07/07 11:00 [entrez] AID - S0006-8993(00)02408-2 [pii] AID - 10.1016/s0006-8993(00)02408-2 [doi] PST - ppublish SO - Brain Res. 2000 Jul 14;871(1):120-6. doi: 10.1016/s0006-8993(00)02408-2.