PMID- 10884510
OWN - NLM
STAT- MEDLINE
DCOM- 20000922
LR  - 20190624
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 399
IP  - 2-3
DP  - 2000 Jul 7
TI  - Neuroprotective activity of chemokines against N-methyl-D-aspartate or 
      beta-amyloid-induced toxicity in culture.
PG  - 117-21
AB  - We have examined the effect of various chemokines on neuronal toxicity in 
      culture. In mixed cortical cultures, challenged with a brief pulse of 
      N-methyl-D-aspartate (NMDA, 60 microM, 10 min), chemokines were either present 
      for 2 h preceding the pulse or they were co-applied with NMDA and then kept in 
      the medium for the following 20-24 h. Interleukin-8 (IL-8), regulated on 
      activation of normal T cells expressed and secreted (RANTES) and 
      macrophage/monocyte chemoattractant protein-1 (MCP-1), were neuroprotective under 
      both conditions, whereas stromal cell-derived factor 1alpha (SDF-1alpha) was 
      protective only when applied during and after the NMDA pulse. Mixed or pure 
      neuronal cultures were also exposed for 48 h to a toxic fragment of the 
      beta-amyloid peptide (beta-amyloid peptide-(25-35), 12.5 or 25 microM) in the 
      absence or presence of chemokines. Among a number of chemokines, only RANTES was 
      neuroprotective against beta-amyloid peptide-(25-35)-induced neurotoxicity in 
      both cultures. We conclude that activation of chemokine receptors differentially 
      affects neuronal degeneration induced by excitotoxins or beta-amyloid peptide in 
      cortical cultures.
FAU - Bruno, V
AU  - Bruno V
AD  - I.N.M. Neuromed, Pozzilli, Italy.
FAU - Copani, A
AU  - Copani A
FAU - Besong, G
AU  - Besong G
FAU - Scoto, G
AU  - Scoto G
FAU - Nicoletti, F
AU  - Nicoletti F
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokines)
RN  - 0 (Interleukin-8)
RN  - 0 (Neuroprotective Agents)
RN  - 6384-92-5 (N-Methylaspartate)
SB  - IM
MH  - Amyloid beta-Peptides/*toxicity
MH  - Animals
MH  - Astrocytes/cytology/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL5/pharmacology
MH  - Chemokines/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Fetus
MH  - Interleukin-8/pharmacology
MH  - Mice
MH  - N-Methylaspartate/*toxicity
MH  - Neurons/cytology/drug effects
MH  - Neuroprotective Agents/*pharmacology
EDAT- 2000/07/08 11:00
MHDA- 2000/09/30 11:01
CRDT- 2000/07/08 11:00
PHST- 2000/07/08 11:00 [pubmed]
PHST- 2000/09/30 11:01 [medline]
PHST- 2000/07/08 11:00 [entrez]
AID - S0014299900003678 [pii]
AID - 10.1016/s0014-2999(00)00367-8 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2000 Jul 7;399(2-3):117-21. doi: 10.1016/s0014-2999(00)00367-8.