PMID- 10886396
OWN - NLM
STAT- MEDLINE
DCOM- 20000718
LR  - 20230412
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 100
IP  - 2
DP  - 2000 Jun
TI  - Efficient induction of an antigen-specific, T helper type 1 immune response by 
      interleukin-12-secreting fibroblasts.
PG  - 203-8
AB  - To determine whether the paracrine secretion of interleukin (IL)-12 can 
      efficiently convert immune responses characterized by high levels of synthesis of 
      IL-4 and immunoglobulin E (IgE) into T helper 1 (Th1)-dominated responses, 3T3 
      fibroblasts were stably transfected to secrete IL-12 (480 units/10(6) cells/48 
      hr). Their effects on the T helper cell-mediated immune response were 
      investigated in ovalbumin (OVA)-primed mice. Free mouse recombinant IL-12 was 
      included as a control group. IL-12-secreting fibroblasts (3T3/IL-12) were more 
      effective than free recombinant IL-12 at increasing OVA-specific interferon-gamma 
      (IFN-gamma) production and decreasing OVA-specific IL-4 production in CD4+ T 
      cells. In addition, injection with 3T3/IL-12 cells significantly increased 
      anti-OVA immunoglobulin G2a (IgG2a) levels and decreased anti-OVA IgE levels in 
      OVA-primed mice. This work suggests that IL-12-secreting fibroblasts can 
      efficiently induce an antigen-specific Th1 response and may be beneficial in the 
      treatment of diseases caused by undesirable T helper 2 (Th2)-dominated responses, 
      including allergic diseases.
FAU - Kim, T S
AU  - Kim TS
AD  - College of Pharmacy and College of Medicine, Chonnam National University, 
      Kwangju, Republic of Korea.
FAU - Kim, K M
AU  - Kim KM
FAU - Shin, B A
AU  - Shin BA
FAU - Hwang, S Y
AU  - Hwang SY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Immunoglobulin G)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Cell Culture Techniques
MH  - Dose-Response Relationship, Immunologic
MH  - Female
MH  - Fibroblasts/*immunology
MH  - Immunization
MH  - Immunoglobulin E/biosynthesis
MH  - Immunoglobulin G/biosynthesis
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-12/*immunology/metabolism
MH  - Interleukin-4/biosynthesis
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/immunology
MH  - Th1 Cells/*immunology
MH  - Transfection
PMC - PMC2327009
EDAT- 2000/07/11 11:00
MHDA- 2000/07/25 11:00
PMCR- 2001/06/01
CRDT- 2000/07/11 11:00
PHST- 2000/07/11 11:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/07/11 11:00 [entrez]
PHST- 2001/06/01 00:00 [pmc-release]
AID - imm038 [pii]
AID - 10.1046/j.1365-2567.2000.00038.x [doi]
PST - ppublish
SO  - Immunology. 2000 Jun;100(2):203-8. doi: 10.1046/j.1365-2567.2000.00038.x.