PMID- 10886563
OWN - NLM
STAT- MEDLINE
DCOM- 20000821
LR  - 20220317
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 58
IP  - 1
DP  - 2000 Jul
TI  - MCP-1 and EGF renal expression and urine excretion in human congenital 
      obstructive nephropathy.
PG  - 182-92
AB  - BACKGROUND: Obstructive nephropathy is characterized at the histologic level by 
      tubular atrophy and interstitial monocyte infiltration. The molecular mechanisms 
      underlying these histologic changes are still poorly defined. Epidermal growth 
      factor (EGF) produced by tubular cells seems to play a pivotal role in the 
      modulation of tubular cell growth, while monocyte chemotactic peptide-1 (MCP-1) 
      is a powerful and specific chemotactic and activating factor for monocytes. 
      METHODS: Twenty-four patients with congenital ureteropelvic junction obstruction 
      [UPJO; 10 with recurrent urinary tract infection (UTI) and 10 with no UTI] and 15 
      healthy children were studied. Diagnosis was made by renal ultrasound, 
      intravenous pielography, and MAG3 scan. Urinary samples were collected before and 
      after surgery. In 10 patients, urine was also collected directly from the 
      affected pelvis at the time of surgery. Urinary EGF and MCP-1 levels were 
      measured by enzyme-linked immunosorbent assay. MCP-1 and EGF gene expression were 
      evaluated by in situ hybridization in 15 biopsies from congenital UPJO and in 10 
      normal kidneys. RESULTS: In normal kidneys, there was a high expression of EGF 
      mRNA, whereas MCP-1 mRNA was undetectable. MCP-1 gene expression was strikingly 
      increased at the tubulointerstitial level in UPJO biopsies compared with controls 
      and was directly correlated with the extent of monocyte infiltration. In 
      addition, UPJO kidney sections showed a marked reduction in EGF gene expression 
      that was directly correlated with the degree of tubular damage. EGF urine 
      concentration was significantly reduced in UPJO when compared with control and 
      directly correlated with its renal gene expression. On the other hand, the MCP-1 
      urine concentration was strikingly increased in UPJO patients. It is noteworthy 
      that a significant and inverse correlation was observed between the MCP-1 
      concentration in the urine collected from the obstructed pelvis and the MAG3 
      clearance of the obstructed kidney (r = -0.76). The presence of recurrent UTI was 
      associated with a significantly higher MCP-1 excretion and a slight reduction in 
      EGF urine concentration. The surgical correction of UPJO was followed by an 
      improvement of renal function together with a significant reduction in MCP-1 
      excretion and a marked increase in EGF urine concentrations. Interestingly, EGF 
      urine concentration measured before surgery was significantly correlated with the 
      difference between the MAG3 clearance of the obstructed kidney before and after 
      surgery. CONCLUSIONS: MCP-1 and EGF seem to be involved in the pathogenesis of 
      tubulointerstitial damage in congenital obstructive nephropathy, and their urine 
      excretion may represent a powerful prognostic marker in this form of renal 
      disease.
FAU - Grandaliano, G
AU  - Grandaliano G
AD  - Division of Nephrology, Department of Emergency and Transplantation University of 
      Bari, Italy.
FAU - Gesualdo, L
AU  - Gesualdo L
FAU - Bartoli, F
AU  - Bartoli F
FAU - Ranieri, E
AU  - Ranieri E
FAU - Monno, R
AU  - Monno R
FAU - Leggio, A
AU  - Leggio A
FAU - Paradies, G
AU  - Paradies G
FAU - Caldarulo, E
AU  - Caldarulo E
FAU - Infante, B
AU  - Infante B
FAU - Schena, F P
AU  - Schena FP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (Biomarkers)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - Adolescent
MH  - Antigens, CD/analysis
MH  - Antigens, Differentiation, Myelomonocytic/analysis
MH  - Biomarkers
MH  - Chemokine CCL2/*genetics/*urine
MH  - Child
MH  - Child, Preschool
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epidermal Growth Factor/*genetics/*urine
MH  - Female
MH  - Gene Expression/physiology
MH  - Humans
MH  - In Situ Hybridization
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Monocytes/cytology
MH  - Prognosis
MH  - RNA, Messenger/analysis
MH  - Radioisotope Renography
MH  - Ureteral Obstruction/congenital/diagnostic imaging/*urine
EDAT- 2000/07/08 11:00
MHDA- 2000/08/29 11:01
CRDT- 2000/07/08 11:00
PHST- 2000/07/08 11:00 [pubmed]
PHST- 2000/08/29 11:01 [medline]
PHST- 2000/07/08 11:00 [entrez]
AID - S0085-2538(15)47087-5 [pii]
AID - 10.1046/j.1523-1755.2000.00153.x [doi]
PST - ppublish
SO  - Kidney Int. 2000 Jul;58(1):182-92. doi: 10.1046/j.1523-1755.2000.00153.x.