PMID- 10887208
OWN - NLM
STAT- MEDLINE
DCOM- 20001103
LR  - 20190513
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 15
IP  - 4
DP  - 2000 Jul
TI  - Spontaneous and X-ray-induced chromosomal aberrations in Werner syndrome cells 
      detected by FISH using chromosome-specific painting probes.
PG  - 303-10
AB  - Werner syndrome (WS) is a rare autosomal disorder characterized by premature 
      aging exhibiting chromosome instability and predisposition to cancer. Cells 
      derived from WS patients show a variety of constitutionally stable chromosomal 
      aberrations as detected by conventional chromosome banding techniques. We have 
      employed the fluorescence in situ hybridization (FISH) technique using painting 
      probes for 12 different chromosomes to detect stable chromosome exchanges in 
      three WS cell lines and three control cell lines. WS cell lines showed increased 
      frequencies of both stable and unstable chromosome aberrations detected by FISH 
      and Giemsa staining, respectively. One WS lymphoblastoid cell line (KO375) had a 
      5/12 translocation in all the cells and approximately 60% of the cells had an 
      additional translocated chromosome 12. A high frequency of aneuploid cells was 
      found in all the WS cell lines studied. Though WS cells are known to be 
      chromosomally unstable, unlike other chromosome instability syndromes they are 
      not sensitive to mutagenic agents. We studied the frequencies of X-ray-induced 
      chromosomal aberrations in two WS cell lines and found an approximately 60% 
      increase in the frequencies of fragments and no consistent increase in the 
      frequencies of exchanges.
FAU - Grigorova, M
AU  - Grigorova M
AD  - Department of Radiation Genetics and Chemical Mutagenesis, Leiden University 
      Medical Centre, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands.
FAU - Balajee, A S
AU  - Balajee AS
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
SB  - IM
MH  - Cell Line
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Chromosome Painting
MH  - Chromosomes/*radiation effects/*ultrastructure
MH  - Chromosomes, Human, Pair 1/radiation effects
MH  - Chromosomes, Human, Pair 12/radiation effects
MH  - Chromosomes, Human, Pair 4/radiation effects
MH  - Chromosomes, Human, Pair 5/radiation effects
MH  - Dose-Response Relationship, Radiation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microscopy, Fluorescence
MH  - Translocation, Genetic
MH  - Werner Syndrome/*genetics
MH  - X-Rays
EDAT- 2000/07/11 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/07/11 11:00
PHST- 2000/07/11 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/07/11 11:00 [entrez]
AID - 10.1093/mutage/15.4.303 [doi]
PST - ppublish
SO  - Mutagenesis. 2000 Jul;15(4):303-10. doi: 10.1093/mutage/15.4.303.