PMID- 10887313
OWN - NLM
STAT- MEDLINE
DCOM- 20000809
LR  - 20061115
IS  - 0091-6749 (Print)
IS  - 0091-6749 (Linking)
VI  - 106
IP  - 1 Pt 1
DP  - 2000 Jul
TI  - Monocyte chemoattractant peptide-1 expression during cutaneous allergic reactions 
      in mice is mast cell dependent and largely mediates the monocyte recruitment 
      response.
PG  - 110-6
AB  - BACKGROUND: Macrophages and infiltrating monocytes comprise the largest 
      population of cells within the airways of patients with allergic asthma. Both 
      become activated and thus contribute to the pathologic features of allergic 
      reactions, but the mechanism by which they are recruited has not been well 
      documented. OBJECTIVE: Our purpose was to assess the role of the CC chemokine 
      monocyte chemotactic peptide-1 (MCP-1) in monocyte recruitment during allergic 
      reactions in mice. METHOD: We used immunohistochemistry and Northern blotting to 
      assess MCP-1 expression, selective mast cell reconstitution of mast 
      cell-deficient W/W(v) mice to demonstrate the mast cell dependence of MCP-1 
      expression and monocyte recruitment and neutralizing anti-MCP-1 antibodies to 
      block monocyte recruitment during cutaneous allergic reactions. RESULTS: MCP-1 
      was expressed largely by resident dermal cells within the allergic lesions at 4 
      hours after challenge, followed within several hours by an influx of monocytes; 
      at 10 hours after challenge monocytes comprised a substantial proportion of the 
      infiltrating cells at the nidus of the response. Mast cell-reconstituted, but not 
      mast cell-deficient W/W(v) mice expressed MCP-1 transcripts and developed 
      monocyte infiltrates after allergen challenge. Finally, anti-MCP-1 antibody 
      treatments reduced by approximately 63% the influx of monocytes into the reaction 
      sites. CONCLUSIONS: These data clearly demonstrate the mast cell dependence of 
      the MCP-1 expression and the monocyte influx and establish a substantial, but not 
      exclusive, causal relationship between these 2 events.
FAU - Gordon, J R
AU  - Gordon JR
AD  - Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, 
      Saskatchewan, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*biosynthesis
MH  - Dermatitis, Allergic Contact/*immunology
MH  - Endothelium/metabolism
MH  - Fibroblasts/metabolism
MH  - Mast Cells/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Monocytes/*immunology
MH  - Skin/cytology
EDAT- 2000/07/11 11:00
MHDA- 2000/08/12 11:00
CRDT- 2000/07/11 11:00
PHST- 2000/07/11 11:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 2000/07/11 11:00 [entrez]
AID - S0091-6749(00)38006-X [pii]
AID - 10.1067/mai.2000.107036 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 2000 Jul;106(1 Pt 1):110-6. doi: 10.1067/mai.2000.107036.