PMID- 10888410
OWN - NLM
STAT- MEDLINE
DCOM- 20001026
LR  - 20190607
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 20
IP  - 1
DP  - 2000 Feb
TI  - Increased stability and catalytic efficiency of yeast hexokinase upon interaction 
      with zwitterionic micelles. Kinetics and conformational studies.
PG  - 41-9
AB  - The effect of ligands (glucose, ATP and Mg2+) and zwitterionic micelles of 
      lysophosphatidylcholine (LPC) or N-hexadecyl-N,N-dimethyl-3-ammonium 
      propanesulfonate (HPS) in the yeast hexokinase (HK) stability was studied at 35 
      degrees C. The thermal inactivation kinetics followed one-exponential decay. The 
      effect of ligands on protecting the enzyme against inactivation followed the 
      order: glucose > glucose/Mg2+ >ATP/Mg2+ approximately or approximately equal to 
      Mg2+l approximately or approximately equal to buffer only. Both LPC and HPS 
      micelles increased the enzyme stability only when the incubation medium contained 
      glucose or glucose/Mg2+, suggesting that the protein conformation is a key 
      prerequisite for the enzyme-micelle interaction to take place. This 
      enzyme-micelle interaction resulted in an increased catalytic efficiency (with a 
      decrease in Km for ATP and increase in Vmax as well as in changes on the tertiary 
      (intrinsic fluorescence) structure of the yeast hexokinase.
FAU - Guerra, R
AU  - Guerra R
AD  - Departamento de Bioquimica Medica, ICB/CCS, Universidade Federal do Rio de 
      Janeiro, CEP, Brazil.
FAU - Bianconi, M L
AU  - Bianconi ML
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (Ligands)
RN  - 0 (Lysophosphatidylcholines)
RN  - 0 (Micelles)
RN  - 0 (N-hexadecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (Surface-Active Agents)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - I38ZP9992A (Magnesium)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adenosine Triphosphate/chemistry
MH  - Catalysis
MH  - Glucose/chemistry
MH  - Hexokinase/*metabolism
MH  - Kinetics
MH  - Ligands
MH  - Lysophosphatidylcholines/pharmacology
MH  - Magnesium/chemistry
MH  - Micelles
MH  - Protein Binding
MH  - Protein Conformation
MH  - Quaternary Ammonium Compounds/pharmacology
MH  - Spectrometry, Fluorescence
MH  - Surface-Active Agents/pharmacology
MH  - Temperature
MH  - Time Factors
MH  - Yeasts/*enzymology
EDAT- 2000/07/11 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/07/11 11:00
PHST- 2000/07/11 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/07/11 11:00 [entrez]
AID - 10.1023/a:1005583117296 [doi]
PST - ppublish
SO  - Biosci Rep. 2000 Feb;20(1):41-9. doi: 10.1023/a:1005583117296.