PMID- 10890176
OWN - NLM
STAT- MEDLINE
DCOM- 20001025
LR  - 20190905
IS  - 0918-8959 (Print)
IS  - 0918-8959 (Linking)
VI  - 47 Suppl
DP  - 2000 Mar
TI  - Serum concentrations of 20K human growth hormone in normal adults and patients 
      with various endocrine disorders. Study Group of 20K hGH.
PG  - S17-21
AB  - The 20K hGH isoform is produced by alternative splicing of GH mRNA, and comprises 
      approximately 10% of all GH in the pituitary. The physiological role of 20K hGH 
      remains to be determined partly because of the lack of a simple and specific 
      assay. We have established sensitive enzyme-linked immunoadsorbent assays 
      (ELISAs) specific to 20K and 22K hGH. The serum levels of 20K hGH after overnight 
      fasting was 118 +/- 178 pg/mL (N=282) in normal women, significantly higher than 
      in normal men (64 +/- 170 pg/mL, N=226). However, there was no difference in the 
      proportion of 20K hGH to 20K plus 22K hGH between men (6.3 +/- 2.6%, N=176) and 
      women (6.3 +/- 2.1%, N = 263). No correlation was detected between the ratio of 
      20K hGH and age, body height, body weight or body fat mass in normal subjects. 
      The proportion of 20K hGH was significantly (P < 0.001) higher in patients with 
      active acromegaly (9.2 +/- 2.2%, N=33) and in patients with anorexia nervosa (9.0 
      +/- 1.9, N=8), both of which are characterized by chronic elevation of 
      circulating GH levels. The proportion of the 20K hGH in successfully treated 
      acromegalic patients did not differ from that in normal subjects, suggesting that 
      GH-producing pituitary tumors secrete a higher proportion of 20K hGH, or chronic 
      excess of 22K hGH altering the metabolic clearance rate of 20K hGH. The values in 
      patients with adult growth hormone deficiency (GHD), hyperthyroidism, primary 
      hypothyroidism, or GH-independent short stature did not differ from those in 
      normal subjects. The 20K ratio did not change after acute GH provocative tests 
      such as insulin tolerance test and GRH test. These results suggest that secretion 
      of 20K hGH from the pituitary is under the same control as that of 22K hGH.
FAU - Tsushima, T
AU  - Tsushima T
AD  - Department of Medicine 2, Tokyo Women's Medical University, Japan.
FAU - Katoh, Y
AU  - Katoh Y
FAU - Miyachi, Y
AU  - Miyachi Y
FAU - Chihara, K
AU  - Chihara K
FAU - Teramoto, A
AU  - Teramoto A
FAU - Irie, M
AU  - Irie M
FAU - Hashimoto, Y
AU  - Hashimoto Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Endocr J
JT  - Endocrine journal
JID - 9313485
RN  - 12629-01-5 (Human Growth Hormone)
SB  - IM
MH  - Acromegaly/blood/therapy
MH  - Adult
MH  - Anorexia Nervosa/blood
MH  - Endocrine System Diseases/*blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - *Genetic Variation
MH  - Human Growth Hormone/*blood/*genetics
MH  - Humans
MH  - Isomerism
MH  - Male
MH  - Molecular Weight
MH  - Osmolar Concentration
MH  - Reference Values
EDAT- 2000/07/13 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/07/13 11:00
PHST- 2000/07/13 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/07/13 11:00 [entrez]
AID - 10.1507/endocrj.47.supplmarch_s17 [doi]
PST - ppublish
SO  - Endocr J. 2000 Mar;47 Suppl:S17-21. doi: 10.1507/endocrj.47.supplmarch_s17.