PMID- 10891580
OWN - NLM
STAT- MEDLINE
DCOM- 20000925
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 78
IP  - 1-2
DP  - 2000 May 31
TI  - Seizures and sensory stimulation result in different patterns of brain derived 
      neurotrophic factor protein expression in the barrel cortex and hippocampus.
PG  - 1-14
AB  - Brain-derived neurotrophic factor (BDNF) is important for the development and 
      trophic support of neurons, and may be involved in controlling axonal sprouting 
      and synaptic plasticity. In order to investigate the activity-dependent 
      regulation of the BDNF gene, BDNF expression was examined within the rat 
      somatosensory cortex (SSC) and hippocampus following vibrissae stimulation, 
      kainic acid induced seizure, and pentylenetetrazol (PTZ) induced seizure. The 
      specific goals of this study were to determine the time course and magnitude of 
      BDNF's activity-dependent expression, and to compare the expression patterns of 
      three commonly used neuronal activation paradigms. Our results demonstrate three 
      novel observations. First, the patterns of BDNF protein expression are dependent 
      upon the neuronal stimulation model used. Both unilateral whisker stimulation (a 
      model of experience dependent plasticity) and kainic acid induced seizure were 
      able to increase the levels of BDNF protein within the SSC and hippocampus. In 
      contrast, PTZ induced seizure did not increase BDNF protein levels in either 
      tissue. Second, there is a dissociation between BDNF mRNA and protein levels 
      following PTZ induced seizure. PTZ seizures resulted in strong increases of BDNF 
      mRNA levels without corresponding increases of the protein. Finally, whisker 
      stimulation resulted in an unexpected increase in BDNF mRNA and protein levels 
      within the hippocampus. These results suggest specific types of neuronal activity 
      can regulate gene expression differently. Furthermore, temporal and spatial 
      differences between the expression of BDNF protein and mRNA levels suggest that 
      the BDNF gene is regulated at the level of translation as well as transcription.
FAU - Nanda, S A
AU  - Nanda SA
AD  - Neuroscience Training Program and Waisman Center, University of Wisconsin, Room 
      707 Waisman Center, 1500 Highland Ave., Madison, WI 53705, USA.
FAU - Mack, K J
AU  - Mack KJ
LA  - eng
GR  - NS33913/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Convulsants)
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (RNA, Messenger)
RN  - SIV03811UC (Kainic Acid)
RN  - WM5Z385K7T (Pentylenetetrazole)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/analysis/*genetics/metabolism
MH  - Convulsants
MH  - Epilepsy/chemically induced/*physiopathology
MH  - Excitatory Amino Acid Agonists
MH  - Gene Expression/physiology
MH  - Hippocampus/chemistry/metabolism/*physiopathology
MH  - Kainic Acid
MH  - Male
MH  - Neuronal Plasticity/physiology
MH  - Pentylenetetrazole
MH  - Physical Stimulation
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seizures/chemically induced/physiopathology
MH  - Somatosensory Cortex/chemistry/metabolism/*physiopathology
MH  - Touch/*physiology
MH  - Vibrissae/innervation/physiology
EDAT- 2000/07/13 11:00
MHDA- 2000/09/30 11:01
CRDT- 2000/07/13 11:00
PHST- 2000/07/13 11:00 [pubmed]
PHST- 2000/09/30 11:01 [medline]
PHST- 2000/07/13 11:00 [entrez]
AID - S0169328X00000541 [pii]
AID - 10.1016/s0169-328x(00)00054-1 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 2000 May 31;78(1-2):1-14. doi: 
      10.1016/s0169-328x(00)00054-1.