PMID- 10893013
OWN - NLM
STAT- MEDLINE
DCOM- 20001107
LR  - 20191104
IS  - 0905-6157 (Print)
IS  - 0905-6157 (Linking)
VI  - 11
IP  - 2
DP  - 2000 May
TI  - Identification of immunogenic maize proteins in a casein hydrolysate formula.
PG  - 106-10
AB  - Cow's milk-based formulas used for infants with cow's milk allergy are based on 
      hydrolyzed proteins. The formulas that are successful in preventing allergic 
      responses are extensively hydrolyzed. Nevertheless, reactions to such formulas 
      are occasionally reported, and protein material of higher molecular weight than 
      expected has been detected by binding immunoglobulin E (IgE) from patients' sera. 
      This paper presents the identification of high-molecular-weight material in the 
      extensively hydrolyzed casein formula, Nutramigen. The material was concentrated 
      by simple centrifugation. The proteins in the pellet were separated by sodium 
      dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and 
      protein-containing bands were analyzed by protein sequencing after 
      electroblotting. The proteins were identified as maize zeins, which are 
      water-insoluble proteins of apparent M(r) 20,000 and 23,000, presumably 
      originating from the maize starch in Nutramigen. Rabbits immunized with this 
      formula developed antibodies against zeins but not against milk proteins. The 
      maize zeins are probably identical to the recently reported components in 
      Nutramigen (1), detected by binding of IgE from milk allergic patients, but not 
      correlated to clinical allergic reactivity. The clinical relevance of maize 
      proteins in Nutramigen remains to be established.
FAU - Frisner, H
AU  - Frisner H
AD  - Department of Biochemistry and Nutrition, Technical University of Denmark, 
      Lyngby.
FAU - Rosendal, A
AU  - Rosendal A
FAU - Barkholt, V
AU  - Barkholt V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Pediatr Allergy Immunol
JT  - Pediatric allergy and immunology : official publication of the European Society 
      of Pediatric Allergy and Immunology
JID - 9106718
RN  - 0 (Antibodies)
RN  - 0 (Caseins)
RN  - 0 (Plant Proteins)
RN  - 0 (Protein Hydrolysates)
SB  - IM
MH  - Animals
MH  - Antibodies/blood/immunology
MH  - Caseins/*chemistry/immunology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - *Infant Food
MH  - Plant Proteins/*immunology
MH  - Protein Hydrolysates/*chemistry/immunology
MH  - Rabbits
MH  - Sequence Analysis, Protein
MH  - Zea mays/chemistry/*immunology
EDAT- 2000/07/13 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/07/13 11:00
PHST- 2000/07/13 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/07/13 11:00 [entrez]
AID - 10.1034/j.1399-3038.2000.00041.x [doi]
PST - ppublish
SO  - Pediatr Allergy Immunol. 2000 May;11(2):106-10. doi: 
      10.1034/j.1399-3038.2000.00041.x.