PMID- 10894887
OWN - NLM
STAT- MEDLINE
DCOM- 20000925
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 452
IP  - 1
DP  - 2000 Jul 20
TI  - Increased nondisjunction of chromosome 21 with age in human peripheral 
      lymphocytes.
PG  - 27-36
AB  - Fluorescence in situ hybridization (FISH) on binucleated cells with 
      chromosome-specific DNA probes provides a convenient way to visualize reciprocal 
      segregation patterns in daughter nuclei, and overcomes most problems related to 
      the artefactual loss or gain of chromosomes that flaw chromosome preparations. In 
      this study, FISH was employed to evaluate age- and sex-effects on spontaneous 
      malsegregation, nondisjunction and loss of chromosome 21 in human lymphocytes 
      after the first division in culture. A total of 68 healthy nonsmokers and 
      nondrinkers of alcohol (37 males and 31 females) were grouped by age as Group I 
      (0-10 years), Group II (20-30 years), Group III (40-50 years) and Group IV (60-70 
      years), with at least seven subjects per group and sex. FISH with a pericentric 
      chromosome 21 specific DNA probe was carried out on binucleated lymphocytes, 
      cytokinesis-blocked by cytochalasin B (6 microg/ml for 26 h) at 44 h after 
      initiation of cultures. Linear regression analyses demonstrated a significant 
      age-related increase in the frequency of micronuclei without chromosome 21 
      (MN-21)(r=0.73, p<0.001 in females; r=0.69, p<0.001 in males) in all binucleated 
      cells, with a steeper slope in females (0.1758) than in males (0. 1241). Analysis 
      using the 2x2 chi-square (chi(2)) test on the frequencies of MN-21 showed 
      significant age-related differences in both males and females, except males in 
      Group III and Group IV (p>0. 05). A significant sex-related difference was found 
      only in subjects over 60 years (p<0.05), with females having more MN-21 (12.57 
      per thousand vs. 8.43 per thousand) than males. Loss of chromosome 21, occurring 
      at mean levels of 0.38 per thousand in all binucleated cells and 0.24 per 
      thousand in binucleated cells containing four FISH signals, was shown not to be 
      age- or sex-related. A positive age-related increase in nondisjunction of 
      chromosome 21 was shown in males (r=0.50, p<0.01), females (r=0.61, p<0.001) and 
      all subjects (r=0.55, p<0.001) by linear regression analysis. An age effect was 
      found only between children and adults (p<0.01 for females, p<0.05
FAU - Shi, Q
AU  - Shi Q
AD  - Institut fuer Saeugetiergenetik, GSF Forschungszentrum fuer Umwelt und Gesundheit 
      GmbH, Neuherberg, Germany.
FAU - Chen, J
AU  - Chen J
FAU - Adler, I
AU  - Adler I
FAU - Zhang, J
AU  - Zhang J
FAU - Martin, R
AU  - Martin R
FAU - Pan, S
AU  - Pan S
FAU - Zhang, X
AU  - Zhang X
FAU - Shan, X
AU  - Shan X
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Age Factors
MH  - Aged
MH  - Cell Nucleus/genetics/pathology
MH  - Child
MH  - Child, Preschool
MH  - Chromosomes, Human, Pair 21/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Infant, Newborn
MH  - Lymphocytes/cytology/*metabolism
MH  - Male
MH  - Micronuclei, Chromosome-Defective
MH  - Middle Aged
MH  - *Nondisjunction, Genetic
EDAT- 2000/07/15 11:00
MHDA- 2000/09/30 11:01
CRDT- 2000/07/15 11:00
PHST- 2000/07/15 11:00 [pubmed]
PHST- 2000/09/30 11:01 [medline]
PHST- 2000/07/15 11:00 [entrez]
AID - S0027510700000324 [pii]
AID - 10.1016/s0027-5107(00)00032-4 [doi]
PST - ppublish
SO  - Mutat Res. 2000 Jul 20;452(1):27-36. doi: 10.1016/s0027-5107(00)00032-4.