PMID- 10894893
OWN - NLM
STAT- MEDLINE
DCOM- 20000925
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 452
IP  - 1
DP  - 2000 Jul 20
TI  - Rapid detection of radiation-induced chromosomal aberrations in lymphocytes and 
      hematopoietic progenitor cells by mFISH.
PG  - 73-81
AB  - Structural chromosome aberrations (SCAs) are sensitive indicators of a preceding 
      exposure of the hematopoietic system to ionizing radiation. Cytogenetic 
      investigations have therefore become routine tools for an assessment of absorbed 
      radiation doses and their biological effects after occupational exposure or 
      radiation accidents. Due to its speed and ease of use, fluorescence in situ 
      hybridization (FISH) with whole chromosome painting (WCP) probes has become a 
      method of choice to visualize SCAs. Until recently, this technique was limited to 
      a rather small number of chromosomes, which could be tested simultaneously. As a 
      result, only a fraction of the structural aberrations present in a sample could 
      be detected and the overall dose effect had to be calculated by extrapolation. 
      The recent introduction of two genome-wide screening techniques in tumor 
      research, i.e., Spectral Karyotyping (SKY) and multicolor FISH (mFISH) now allows 
      the detection of translocations involving any two non-homologous chromosomes. The 
      present study was prompted by our desire to bring the power of mFISH to bear for 
      the rapid identification of radiation-induced SCAs. We chose two model systems to 
      investigate the utility of mFISH: lymphocytes that were exposed in vitro to 3 Gy 
      photons and single hematopoietic progenitor cell colonies isolated from a 
      Chernobyl victim 9 years after in vivo exposure to 5.4 Sv.In lymphocytes, we 
      found up to 15 different chromosomes involved in rearrangements indicating 
      complex radiation effects. Stable aberrations detected in hematopoietic cell 
      colonies, on the other hand, showed involvement of up to three different 
      chromosomes. These results demonstrated that mFISH is a rapid and powerful 
      approach to detect and characterize radiation-induced SCAs in the hemopoietic 
      system. The application of mFISH is expected to result in a more detailed and, 
      thus, more informative picture of radiation effects. Eventually, this technique 
      will allow researchers to rapidly delineate chromosomal breakpoints and 
      facilitate the identification of the genes involved in radiation tumorigenesis.
FAU - Greulich, K M
AU  - Greulich KM
AD  - Department of Radiation Oncology, Technical University of Munich, Germany. 
      kgreulich@primus-online.de
FAU - Kreja, L
AU  - Kreja L
FAU - Heinze, B
AU  - Heinze B
FAU - Rhein, A P
AU  - Rhein AP
FAU - Weier, H G
AU  - Weier HG
FAU - Bruckner, M
AU  - Bruckner M
FAU - Fuchs, P
AU  - Fuchs P
FAU - Molls, M
AU  - Molls M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
SB  - IM
MH  - Adult
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Chromosome Painting
MH  - Female
MH  - Hematopoietic Stem Cells/cytology/metabolism/*radiation effects
MH  - Humans
MH  - Karyotyping
MH  - Lymphocytes/cytology/metabolism/*radiation effects
MH  - Male
EDAT- 2000/07/15 11:00
MHDA- 2000/09/30 11:01
CRDT- 2000/07/15 11:00
PHST- 2000/07/15 11:00 [pubmed]
PHST- 2000/09/30 11:01 [medline]
PHST- 2000/07/15 11:00 [entrez]
AID - S0027510700000579 [pii]
AID - 10.1016/s0027-5107(00)00057-9 [doi]
PST - ppublish
SO  - Mutat Res. 2000 Jul 20;452(1):73-81. doi: 10.1016/s0027-5107(00)00057-9.