PMID- 10896131
OWN - NLM
STAT- MEDLINE
DCOM- 20001108
LR  - 20061115
IS  - 1434-4610 (Print)
IS  - 1434-4610 (Linking)
VI  - 151
IP  - 1
DP  - 2000 May
TI  - Investigations on gene copy number, introns and chromosomal arrangement of genes 
      encoding the fucoxanthin chlorophyll a/c-binding proteins of the centric diatom 
      Cyclotella cryptica.
PG  - 27-39
AB  - The gene arrangement, existence of introns and the number of gene copies of genes 
      (fcps) encoding fucoxanthin chlorophyll a/c-binding proteins (Fcps) of the 
      centric diatom Cyclotella cryptica were investigated by polymerase chain reaction 
      (PCR), Southern blotting and denaturing gradient gel electrophoresis (DGGE) 
      experiments. PCR-mediated amplification of the fcp genes using chromosomal DNA as 
      template demonstrated the absence of introns within the amplified regions. 
      Clustering of genes could not be demonstrated in these experiments. Digestion of 
      chromosomal DNA of Cy. cryptica followed by Southern blotting and hybridization 
      with specific fcp probes revealed minimum and maximum values of 12 and 20, 
      respectively, for the gene copies. In addition, the DGGE technique confirmed and 
      strengthened the results obtained from Southern blotting experiments as 
      amplification of gene fragments from genomic DNA with different sets of specific 
      primers revealed values of 21 and 23, for the minimum and maximum gene copy 
      number, respectively.
FAU - Eppard, M
AU  - Eppard M
AD  - AG Geomikrobiologie, ICBM, Carl-von-Ossietzky-Universitat Oldenburg, Germany.
FAU - Rhiel, E
AU  - Rhiel E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Protist
JT  - Protist
JID - 9806488
RN  - 0 (Light-Harvesting Protein Complexes)
RN  - 0 (Photosynthetic Reaction Center Complex Proteins)
RN  - 0 (fucoxanthin-, chlorophyll a-c-containing protein)
SB  - IM
MH  - Blotting, Southern
MH  - Chromosomes
MH  - Diatoms/*genetics
MH  - Electrophoresis/methods
MH  - *Gene Dosage
MH  - Introns
MH  - *Light-Harvesting Protein Complexes
MH  - Multigene Family
MH  - Photosynthetic Reaction Center Complex Proteins/*genetics
EDAT- 2000/07/15 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/07/15 11:00
PHST- 2000/07/15 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/07/15 11:00 [entrez]
AID - S1434-4610(04)70005-X [pii]
AID - 10.1078/1434-4610-00005 [doi]
PST - ppublish
SO  - Protist. 2000 May;151(1):27-39. doi: 10.1078/1434-4610-00005.