PMID- 10899220
OWN - NLM
STAT- MEDLINE
DCOM- 20000907
LR  - 20181113
IS  - 0022-3077 (Print)
IS  - 1522-1598 (Electronic)
IS  - 0022-3077 (Linking)
VI  - 84
IP  - 1
DP  - 2000 Jul
TI  - BDNF-Induced potentiation of spontaneous twitching in innervated myocytes 
      requires calcium release from intracellular stores.
PG  - 472-83
AB  - Brain-derived neurotrophic factor (BDNF) can potentiate synaptic release at newly 
      developed frog neuromuscular junctions. Although this potentiation depends on 
      extracellular Ca(2+) and reflects changes in acetylcholine release, little is 
      known about the intracellular transduction or calcium signaling pathways. We have 
      developed a video assay for neurotrophin-induced potentiation of myocyte 
      twitching as a measure of potentiation of synaptic activity. We use this assay to 
      show that BDNF-induced synaptic potentiation is not blocked by cadmium, 
      indicating that Ca(2+) influx through voltage-gated Ca(2+) channels is not 
      required. TrkB autophosphorylation is not blocked in Ca(2+)-free conditions, 
      indicating that TrkB activity is not Ca(2+) dependent. Additionally, an inhibitor 
      of phospholipase C interferes with BDNF-induced potentiation. These results 
      suggest that activation of the TrkB receptor activates phospholipase C to 
      initiate intracellular Ca(2+) release from stores which subsequently potentiates 
      transmitter release.
FAU - Kleiman, R J
AU  - Kleiman RJ
AD  - Department of Physiology, University of California, San Francisco, California 
      94143-0723, USA.
FAU - Tian, N
AU  - Tian N
FAU - Krizaj, D
AU  - Krizaj D
FAU - Hwang, T N
AU  - Hwang TN
FAU - Copenhagen, D R
AU  - Copenhagen DR
FAU - Reichardt, L F
AU  - Reichardt LF
LA  - eng
GR  - P01 NS016033/NS/NINDS NIH HHS/United States
GR  - P01 NS016033-17A10014/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurophysiol
JT  - Journal of neurophysiology
JID - 0375404
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calcium Channels)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Estrenes)
RN  - 0 (Indoles)
RN  - 0 (Phosphodiesterase Inhibitors)
RN  - 0 (Pyrrolidinones)
RN  - 00BH33GNGH (Cadmium)
RN  - 112648-68-7 
      (1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - 42HK56048U (Tyrosine)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - SY7Q814VUP (Calcium)
RN  - X9TLY4580Z (cyclopiazonic acid)
SB  - IM
MH  - Action Potentials/physiology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cadmium/pharmacology
MH  - Calcium/*metabolism/pharmacokinetics
MH  - Calcium Channels/physiology
MH  - Enzyme Inhibitors/pharmacology
MH  - Estrenes/pharmacology
MH  - Excitatory Postsynaptic Potentials/drug effects/physiology
MH  - Indoles/pharmacology
MH  - Motor Neurons/*physiology
MH  - Muscle Contraction/drug effects/physiology
MH  - Muscle Fibers, Skeletal/*drug effects/*physiology
MH  - Oocytes/physiology
MH  - PC12 Cells
MH  - Phosphodiesterase Inhibitors/pharmacology
MH  - Phosphorylation
MH  - Phosphotyrosine/analysis
MH  - Pyrrolidinones/pharmacology
MH  - Rats
MH  - Receptor, trkB/physiology
MH  - Synapses/enzymology
MH  - Thapsigargin/pharmacology
MH  - Type C Phospholipases/antagonists & inhibitors/metabolism
MH  - Tyrosine/metabolism
MH  - Xenopus
PMC - PMC2710114
MID - NIHMS113042
EDAT- 2000/07/19 11:00
MHDA- 2000/09/09 11:01
PMCR- 2009/07/14
CRDT- 2000/07/19 11:00
PHST- 2000/07/19 11:00 [pubmed]
PHST- 2000/09/09 11:01 [medline]
PHST- 2000/07/19 11:00 [entrez]
PHST- 2009/07/14 00:00 [pmc-release]
AID - 10.1152/jn.2000.84.1.472 [doi]
PST - ppublish
SO  - J Neurophysiol. 2000 Jul;84(1):472-83. doi: 10.1152/jn.2000.84.1.472.