PMID- 10899974
OWN - NLM
STAT- MEDLINE
DCOM- 20010105
LR  - 20240109
IS  - 1040-4651 (Print)
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Linking)
VI  - 12
IP  - 7
DP  - 2000 Jul
TI  - Light-induced nuclear translocation of endogenous pea phytochrome A visualized by 
      immunocytochemical procedures.
PG  - 1063-78
AB  - Although the physiological functions of phytochrome A (PhyA) are now known, the 
      distribution of endogenous PhyA has not been examined. We have visualized 
      endogenous PhyA apoprotein (PHYA) by immunolabeling cryosections of pea tissue, 
      using PHYA-deficient mutants as negative controls. By this method, we examined 
      the distribution of PHYA in different tissues and changes in its intracellular 
      distribution in response to light. In apical hook cells of etiolated seedlings, 
      PHYA immunolabeling was distributed diffusely in the cytosol. Exposure to 
      continuous far-red (cFR) light caused a redistribution of the immunolabeling to 
      the nucleus, first detectable after 1.5 hr and greatest at 4.5 hr. During this 
      time, the amounts of spectrally active phytochrome and PHYA did not decline 
      substantially. Exposure to continuous red (cR) light or to a brief pulse of red 
      light also resulted in redistribution of immunolabeling to the nucleus, but this 
      occurred much more rapidly and with a different pattern of intranuclear 
      distribution than it did in response to cFR light. Exposures to cR light resulted 
      in loss of immunolabeling, which was associated with PHYA degradation. These 
      results indicate that the light-induced intracellular location of PHYA is 
      wavelength dependent and imply that this is important for PhyA activity.
FAU - Hisada, A
AU  - Hisada A
AD  - Hitachi Advanced Research Laboratory, Hatoyama, Saitama, Japan.
FAU - Hanzawa, H
AU  - Hanzawa H
FAU - Weller, J L
AU  - Weller JL
FAU - Nagatani, A
AU  - Nagatani A
FAU - Reid, J B
AU  - Reid JB
FAU - Furuya, M
AU  - Furuya M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Phytochrome A)
RN  - 11121-56-5 (Phytochrome)
SB  - IM
MH  - Biological Transport
MH  - Cell Nucleus/*metabolism
MH  - Darkness
MH  - Immunohistochemistry
MH  - Pisum sativum/metabolism/*radiation effects
MH  - Phytochrome/*metabolism
MH  - Phytochrome A
PMC - PMC149049
EDAT- 2000/07/19 11:00
MHDA- 2001/02/28 10:01
PMCR- 2001/07/01
CRDT- 2000/07/19 11:00
PHST- 2000/07/19 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/07/19 11:00 [entrez]
PHST- 2001/07/01 00:00 [pmc-release]
AID - 99-0428 [pii]
AID - 10.1105/tpc.12.7.1063 [doi]
PST - ppublish
SO  - Plant Cell. 2000 Jul;12(7):1063-78. doi: 10.1105/tpc.12.7.1063.