PMID- 10901128
OWN - NLM
STAT- MEDLINE
DCOM- 20001101
LR  - 20190831
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 742
IP  - 2
DP  - 2000 Jun 9
TI  - High-performance liquid chromatographic analysis of AQ4N, an 
      alkylaminoanthraquinone N-oxide.
PG  - 239-45
AB  - A simple, highly selective and reproducible reversed-phase high-performance 
      liquid chromatography method has been developed for the analysis of the new 
      anti-cancer pro-drug AQ4N. The sample pre-treatment involves a simple protein 
      precipitation protocol, using methanol. Chromatographic separations were 
      performed using a HiChrom HIRPB (25 cmX4.6 mm I.D.) column, with mobile phase of 
      acetonitrile-ammonium formate buffer (0.05 M) (22:78, v/v), with final pH 
      adjusted to 3.6 with formic acid. The flow-rate was maintained at 1.2 ml min(-1). 
      Detection was via photodiode array performed in the UV range at 242 nm and, since 
      the compounds are an intense blue colour, in the visible range at 612 nm. The 
      structurally related compound mitoxantrone was used as internal standard. The 
      validated quantification range of the method was 0.05-10.0 microg ml(-1) in mouse 
      plasma. The inter-day relative standard deviations (RSDs) (n=5) ranged from 18.4% 
      and 12.1% at 0.05 microg ml(-1) to 2.9% and 3.3% at 10.0 microg ml(-1) for AQ4N 
      and AQ4, respectively. The intra-day RSDs for supplemented mouse plasma (n=6) 
      ranged from 8.2% and 14.2% at 0.05 microg ml(-1) to 7.6% and 11.5% at 10.0 microg 
      ml(-1) for AQ4N and AQ4, respectively. The overall recovery of the procedure for 
      AQ4N was 89.4 +/- 1.77% and 76.1 +/- 7.26% for AQ4. The limit of detection was 50 
      ng ml(-1) with a 100 microl sample volume. The method described provides a 
      suitable technique for the future analysis of low levels of AQ4N and AQ4 in 
      clinical samples.
FAU - Swaine, D J
AU  - Swaine DJ
AD  - Clinical Oncology Unit, University of Bradford, West Yorkshire, UK.
FAU - Loadman, P M
AU  - Loadman PM
FAU - Bibby, M C
AU  - Bibby MC
FAU - Graham, M A
AU  - Graham MA
FAU - Patterson, L H
AU  - Patterson LH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 0 (Anthraquinones)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Blood Proteins)
RN  - 0 (Prodrugs)
RN  - 658876KMFP (AQ4N)
SB  - IM
MH  - Animals
MH  - Anthraquinones/*blood/pharmacokinetics
MH  - Antineoplastic Agents/*blood/pharmacokinetics
MH  - Blood Proteins/metabolism
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Female
MH  - Mice
MH  - Prodrugs/*pharmacokinetics
MH  - Protein Binding
MH  - Reference Standards
EDAT- 2000/07/20 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/07/20 11:00
PHST- 2000/07/20 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/07/20 11:00 [entrez]
AID - 10.1016/s0378-4347(00)00160-2 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 2000 Jun 9;742(2):239-45. doi: 
      10.1016/s0378-4347(00)00160-2.