PMID- 10902721
OWN - NLM
STAT- MEDLINE
DCOM- 20001129
LR  - 20190915
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 43
IP  - 3
DP  - 2000 Jun
TI  - Molecular analyses of a repetitive DNA sequence in wheat (Triticum aestivum L.).
PG  - 556-63
AB  - A repetitive sequence designated WE35 was isolated from wheat genomic DNA. This 
      sequence consists of a 320-bp repeat unit and represents approximately 0.002% of 
      the total wheat DNA. It is unidirectionally distributed either continuously or 
      discretely in the genome. Ladder-like banding patterns were observed in Southern 
      blots when the wheat genomic DNA was restricted with endonuclease enzymes EcoRI, 
      HincII, NciI, and NdeI, which is characteristic for tandemly organized sequences. 
      Two DNA fragments in p451 were frequently associated with the WE35 repetitive 
      unit in a majority of lambda wheat genomic clones. A 475-bp fragment homologous 
      to the 5'-end long terminal repeat (LTR) of cereal retroelements was also found 
      in some lambda wheat genomic clones containing the repetitive unit. Physical 
      mapping by fluorescence in situ hybridization (FISH) indicated that one pair of 
      wheat chromosomes could be specifically detected with the WE35 positive probe 
      p551. WE35 can be considered a chromosome-specific repetitive sequence. This 
      repetitive unit could be used as a molecular marker for genetic, phylogenetic, 
      and evolutionary studies in the tribe Triticeae.
FAU - Ueng, P P
AU  - Ueng PP
AD  - Molecular Plant Pathology Lab, USDA-ARS, Beltsville, MD 20705, USA. 
      pueng@asrr.arsusda.gov
FAU - Hang, A
AU  - Hang A
FAU - Tsang, H
AU  - Tsang H
FAU - Vega, J M
AU  - Vega JM
FAU - Wang, L
AU  - Wang L
FAU - Burton, C S
AU  - Burton CS
FAU - He, F T
AU  - He FT
FAU - Liu, B
AU  - Liu B
LA  - eng
SI  - GENBANK/AF087828
SI  - GENBANK/AF139201
SI  - GENBANK/AF139202
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Chromosome Mapping
MH  - DNA, Plant/*genetics/isolation & purification
MH  - *Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Triticum/*genetics/ultrastructure
EDAT- 2000/07/21 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/07/21 11:00
PHST- 2000/07/21 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/07/21 11:00 [entrez]
AID - 10.1139/g99-143 [doi]
PST - ppublish
SO  - Genome. 2000 Jun;43(3):556-63. doi: 10.1139/g99-143.