PMID- 10902816
OWN - NLM
STAT- MEDLINE
DCOM- 20000808
LR  - 20051117
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 85
IP  - 7
DP  - 2000 Jul
TI  - Expression of menin in parathyroid tumors.
PG  - 2615-9
AB  - The multiple endocrine neoplasia type 1 (MEN1) gene seems to be a tumor 
      suppressor that encodes a 610-amino acid protein termed menin and that plays an 
      important role in the development of MEN1 syndrome. Recent reports indicate that 
      heterozygous germline mutations of this gene are responsible for the disease 
      onset of MEN1. In this study we examined the expression of menin in parathyroid 
      tumors from primary hyperparathyroidism (PHP), secondary hyperparathyroidism 
      (SHP), and MEN1 and thyroid tumors including Basedow's disease, thyroid cancer, 
      and adrenocortical tumors. Both ribonucleic acid and protein from these tumors 
      were applied to RT-PCR and Western blotting, respectively. Primers for RT-PCR 
      were designed to amplify the sequence between exons 2 and 3 of the MEN1 gene. 
      Specific antibody against menin was generated in guinea pigs immunized with the 
      recombinant peptide from amino acid residues 443-535 of menin made by using 
      glutathione-S-transferase (GST) gene fusion. Menin messenger ribonucleic acid was 
      strongly expressed on RT-PCR analysis in the parathyroid tumors from both PHP and 
      SHP. Western blotting revealed a specific band of approximately 67 kDa in 
      parathyroid tumors from PHP and SHP, with a much weaker such band detected in 
      thyroid tumors. Menin expression was down-regulated in MEN1 samples, including 
      nonsense mutation and deletion mutant. These findings suggest that menin is 
      predominantly synthesized and stored in parathyroid tumors resulting from PHP and 
      SHP.
FAU - Bhuiyan, M M
AU  - Bhuiyan MM
AD  - First Department of Internal Medicine, Kagawa Medical University, Japan.
FAU - Sato, M
AU  - Sato M
FAU - Murao, K
AU  - Murao K
FAU - Imachi, H
AU  - Imachi H
FAU - Namihira, H
AU  - Namihira H
FAU - Takahara, J
AU  - Takahara J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Actins)
RN  - 0 (DNA Primers)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
SB  - IM
MH  - Actins/biosynthesis/genetics
MH  - Adrenal Gland Neoplasms/metabolism
MH  - Artificial Gene Fusion
MH  - Blotting, Western
MH  - DNA Primers
MH  - Genes, Tumor Suppressor/*genetics
MH  - Humans
MH  - Neoplasm Proteins/*biosynthesis/genetics
MH  - Parathyroid Neoplasms/*metabolism
MH  - *Proto-Oncogene Proteins
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Thyroid Neoplasms/metabolism
EDAT- 2000/07/21 11:00
MHDA- 2000/08/12 11:00
CRDT- 2000/07/21 11:00
PHST- 2000/07/21 11:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 2000/07/21 11:00 [entrez]
AID - 10.1210/jcem.85.7.6688 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 2000 Jul;85(7):2615-9. doi: 10.1210/jcem.85.7.6688.