PMID- 10905579
OWN - NLM
STAT- MEDLINE
DCOM- 20001207
LR  - 20190817
IS  - 0168-8278 (Print)
IS  - 0168-8278 (Linking)
VI  - 33
IP  - 1
DP  - 2000 Jul
TI  - Downregulation of ileal bile acid absorption in bile-duct-ligated rats.
PG  - 2-8
AB  - BACKGROUND/AIMS: Accumulation of toxic bile acids in cholestasis contributes to 
      liver injury and depends on their synthesis, secretion and intestinal absorption. 
      In the present study, we investigated the effect of cholestasis on the active 
      ileal absorption of bile acids in vivo and the adaptation of transporters 
      involved in ileal bile acid absorption. METHODS: Male Wistar rats underwent 
      ligation of the common bile duct or biliary diversion. Sham-operated rats served 
      as controls. Active ileal bile acid absorption of taurocholate was measured by an 
      intestinal perfusion technique. Transporter mRNA levels of the Na+/bile acid 
      cotransporting protein (IBAT), ileal lipid binding protein (ILBP) and organic 
      anion transporter subtype 3 (Oatp3) and protein expression of IBAT and ILBP were 
      determined in the distal ileum. RESULTS: After bile duct ligation the intestinal 
      absorption rates of taurocholate were lower (p<0.05) and after biliary diversion 
      absorption rates were higher compared to sham-operated animals (p<0.05). The 
      absorption rates were inversely correlated to serum bile acid concentrations. 
      Levels of IBAT-, ILBP- and Oatp3- mRNA were not different between the groups. 
      However, in cholestatic rats, the expression of the 99-kDa dimer of IBAT was 
      decreased compared to controls (p<0.05), whereas the 46-kDa monomeric protein of 
      IBAT and the expression of ILBP was unchanged. After biliary diversion a similar 
      pattern of protein expression was observed, despite an increased absorption rate. 
      CONCLUSIONS: Cholestasis leads to a decreased active ileal absorption of 
      taurocholate. The changes in protein expression may not account for the different 
      absorption rates. The intestinal absorption of bile acids seems to be regulated 
      by their systemic concentration.
FAU - Sauer, P
AU  - Sauer P
AD  - Department of Medicine, University of Heidelberg, Germany.
FAU - Stiehl, A
AU  - Stiehl A
FAU - Fitscher, B A
AU  - Fitscher BA
FAU - Riedel, H D
AU  - Riedel HD
FAU - Benz, C
AU  - Benz C
FAU - Kloters-Plachky, P
AU  - Kloters-Plachky P
FAU - Stengelin, S
AU  - Stengelin S
FAU - Stremmel, W
AU  - Stremmel W
FAU - Kramer, W
AU  - Kramer W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Hepatol
JT  - Journal of hepatology
JID - 8503886
RN  - 0 (Carrier Proteins)
RN  - 0 (Organic Anion Transporters, Sodium-Dependent)
RN  - 0 (Organic Anion Transporters, Sodium-Independent)
RN  - 0 (RNA, Messenger)
RN  - 0 (Symporters)
RN  - 0 (organic anion transport protein 3)
RN  - 145420-23-1 (sodium-bile acid cotransporter)
RN  - 5E090O0G3Z (Taurocholic Acid)
SB  - IM
MH  - Animals
MH  - Bile Ducts
MH  - Biliopancreatic Diversion
MH  - Carrier Proteins/chemistry/genetics
MH  - Cholestasis, Extrahepatic/etiology/*metabolism
MH  - Dimerization
MH  - Down-Regulation
MH  - Ileum/*metabolism
MH  - Intestinal Absorption
MH  - Ligation
MH  - Male
MH  - *Organic Anion Transporters, Sodium-Dependent
MH  - *Organic Anion Transporters, Sodium-Independent
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reference Values
MH  - *Symporters
MH  - Taurocholic Acid/*metabolism
EDAT- 2000/07/25 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/07/25 11:00
PHST- 2000/07/25 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/07/25 11:00 [entrez]
AID - S0168-8278(00)80152-X [pii]
AID - 10.1016/s0168-8278(00)80152-x [doi]
PST - ppublish
SO  - J Hepatol. 2000 Jul;33(1):2-8. doi: 10.1016/s0168-8278(00)80152-x.