PMID- 10906434
OWN - NLM
STAT- MEDLINE
DCOM- 20000824
LR  - 20190819
IS  - 0378-4274 (Print)
IS  - 0378-4274 (Linking)
VI  - 116
IP  - 1-2
DP  - 2000 Jul 27
TI  - Evaluation of skin sensitization potential of jet fuels by murine local lymph 
      node assay.
PG  - 165-70
AB  - Jet A and JP-8 are the major jet fuels used in civilian and military (US Air 
      Force) flights, respectively. JP-8+100 is a new jet fuel recently introduced by 
      the US Air Force. Besides lung exposure, skin is the potential route of exposure 
      to jet fuels. The purpose of the present study was to investigate the skin 
      sensitization potential of jet fuels (Jet A, JP-8 and JP-8+100) using murine 
      Local lymph node assay (LLNA). Female CBA/Ca mice (8-12-weeks-old) were used in 
      the study. Dinitrochlorobenzene (DNCB, 0.25% w/v) and paraaminobenzoic acid 
      (PABA, 2.5% w/v) were used as positive and negative control, respectively and 
      acetone: olive oil (4:1, AOO) was used as the vehicle (control). All three jet 
      fuels caused a proliferative activity significantly greater than the control 
      (P<0.01). Our results demonstrate that JP-8 is a weak skin sensitizer 
      [stimulation index (SI)=3.17]. The SI of Jet A and JP-8+100 were 2.44 and 2.38, 
      respectively, hence are not considered as skin sensitizers. Interestingly, the SI 
      of JP-8 with butylated hydroxytoluene (BHT) was consistently lower than JP-8, 
      though the difference was not statistically significant (P>0.05). BHT, which is 
      an antioxidant additive of JP-8+100, reduced the skin sensitization potential of 
      JP-8. Furthermore, the lower SI of JP-8+100 could be partially attributed to the 
      presence of BHT. The findings reported here suggest that care should be taken to 
      minimize dermal exposure to jet fuels especially JP-8 to avoid skin 
      sensitization.
FAU - Kanikkannan, N
AU  - Kanikkannan N
AD  - Division of Pharmaceutics, College of Pharmacy, Florida A & M University, 
      Tallahassee 32307, USA.
FAU - Jackson, T
AU  - Jackson T
FAU - Sudhan Shaik, M
AU  - Sudhan Shaik M
FAU - Singh, M
AU  - Singh M
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Hydrocarbons)
RN  - 0 (JP8 aviation fuel)
RN  - 0 (Kerosene)
RN  - 25013-16-5 (Butylated Hydroxyanisole)
SB  - IM
MH  - Animals
MH  - Butylated Hydroxyanisole/toxicity
MH  - Female
MH  - Hydrocarbons/*toxicity
MH  - Kerosene/*toxicity
MH  - Lymph Nodes/*drug effects
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Skin/*drug effects
EDAT- 2000/07/25 11:00
MHDA- 2000/08/29 11:01
CRDT- 2000/07/25 11:00
PHST- 2000/07/25 11:00 [pubmed]
PHST- 2000/08/29 11:01 [medline]
PHST- 2000/07/25 11:00 [entrez]
AID - S0378-4274(00)00212-5 [pii]
AID - 10.1016/s0378-4274(00)00212-5 [doi]
PST - ppublish
SO  - Toxicol Lett. 2000 Jul 27;116(1-2):165-70. doi: 10.1016/s0378-4274(00)00212-5.