PMID- 10910900
OWN - NLM
STAT- MEDLINE
DCOM- 20000824
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 96
IP  - 3
DP  - 2000 Aug 1
TI  - In vivo generation of human dendritic cell subsets by Flt3 ligand.
PG  - 878-84
AB  - Dendritic cells (DCs) represent a family of ontogenically distinct leukocytes 
      involved in immune response regulation. The ability of DCs to stimulate T-cell 
      immunity has led to their use as vectors for immunotherapy vaccines. However, it 
      is unclear whether and to what degree in vitro-generated DCs are representative 
      of DCs that develop in vivo. Treatment of mice with human Flt3 ligand (FL) 
      dramatically increases the number of DCs. We report here that administration of 
      FL to healthy human volunteers increased the number of circulating CD11c(+ 
      )IL-3Ralpha(low) DC (mean 44-fold) and CD11c(-) IL-3Ralpha(high) DC precursors 
      (mean 12-fold). Moreover, the CD11c(+ )DCs were efficient stimulators of T cells 
      in vitro. Thus, FL can expand the number of circulating, functionally competent 
      human DCs in vivo.
FAU - Maraskovsky, E
AU  - Maraskovsky E
AD  - Immunex Corporation, Seattle, WA, USA. eugene.maraskovsky@ludwig.edu.au
FAU - Daro, E
AU  - Daro E
FAU - Roux, E
AU  - Roux E
FAU - Teepe, M
AU  - Teepe M
FAU - Maliszewski, C R
AU  - Maliszewski CR
FAU - Hoek, J
AU  - Hoek J
FAU - Caron, D
AU  - Caron D
FAU - Lebsack, M E
AU  - Lebsack ME
FAU - McKenna, H J
AU  - McKenna HJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Ligands)
RN  - 0 (Membrane Proteins)
RN  - 0 (flt3 ligand protein)
SB  - IM
MH  - Adjuvants, Immunologic/administration & dosage
MH  - Cell Differentiation/drug effects/immunology
MH  - Dendritic Cells/cytology/*immunology
MH  - Humans
MH  - *Immunity, Cellular
MH  - Ligands
MH  - Membrane Proteins/administration & dosage/*immunology
MH  - T-Lymphocytes/immunology
EDAT- 2000/07/27 11:00
MHDA- 2000/08/29 11:01
CRDT- 2000/07/27 11:00
PHST- 2000/07/27 11:00 [pubmed]
PHST- 2000/08/29 11:01 [medline]
PHST- 2000/07/27 11:00 [entrez]
AID - S0006-4971(20)72012-4 [pii]
PST - ppublish
SO  - Blood. 2000 Aug 1;96(3):878-84.