PMID- 10913178
OWN - NLM
STAT- MEDLINE
DCOM- 20000828
LR  - 20240407
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 20
IP  - 16
DP  - 2000 Aug
TI  - A U-rich element in the 5' untranslated region is necessary for the translation 
      of p27 mRNA.
PG  - 5947-59
AB  - Increased translation of p27 mRNA correlates with withdrawal of cells from the 
      cell cycle. This raised the possibility that antimitogenic signals might mediate 
      their effects on p27 expression by altering complexes that formed on p27 mRNA, 
      regulating its translation. In this report, we identify a U-rich sequence in the 
      5' untranslated region (5'UTR) of p27 mRNA that is necessary for efficient 
      translation in proliferating and nonproliferating cells. We show that a number of 
      factors bind to the 5'UTR in vitro in a manner dependent on the U-rich element, 
      and their availability in the cytosol is controlled in a growth- and cell 
      cycle-dependent fashion. One of these factors is HuR, a protein previously 
      implicated in mRNA stability, transport, and translation. Another is hnRNP C1 and 
      C2, proteins implicated in mRNA processing and the translation of a specific 
      subset of mRNAs expressed in differentiated cells. In lovastatin-treated MDA468 
      cells, the mobility of the associated hnRNP C1 and C2 proteins changed, and this 
      correlated with increased p27 expression. Together, these data suggest that the 
      U-rich dependent RNP complex on the 5'UTR may regulate the translation of p27 
      mRNA and may be a target of antimitogenic signals.
FAU - Millard, S S
AU  - Millard SS
AD  - Graduate Program in Cell Biology and Genetics, Weill Graduate School of Medical 
      Sciences, Cornell University, New York, New York 10021, USA.
FAU - Vidal, A
AU  - Vidal A
FAU - Markus, M
AU  - Markus M
FAU - Koff, A
AU  - Koff A
LA  - eng
GR  - P30 CA008748/CA/NCI NIH HHS/United States
GR  - R01 GM052597/GM/NIGMS NIH HHS/United States
GR  - CA08748/CA/NCI NIH HHS/United States
GR  - GM52597/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tagln protein, mouse)
SB  - IM
MH  - 5' Untranslated Regions/*genetics
MH  - Base Sequence
MH  - Cell Cycle/genetics
MH  - HeLa Cells
MH  - Humans
MH  - Microfilament Proteins/*genetics
MH  - Molecular Sequence Data
MH  - *Muscle Proteins
MH  - *Protein Biosynthesis
MH  - RNA, Messenger/*genetics
PMC - PMC86072
EDAT- 2000/07/27 11:00
MHDA- 2000/09/02 11:01
PMCR- 2000/08/01
CRDT- 2000/07/27 11:00
PHST- 2000/07/27 11:00 [pubmed]
PHST- 2000/09/02 11:01 [medline]
PHST- 2000/07/27 11:00 [entrez]
PHST- 2000/08/01 00:00 [pmc-release]
AID - 0562 [pii]
AID - 10.1128/MCB.20.16.5947-5959.2000 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2000 Aug;20(16):5947-59. doi: 10.1128/MCB.20.16.5947-5959.2000.