PMID- 10913673
OWN - NLM
STAT- MEDLINE
DCOM- 20000809
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 120
IP  - 1
DP  - 2000 Jul 1
TI  - Interphase FISH for Y chromosome, VNTR polymorphisms, and RT-PCR for BCR-ABL in 
      the monitoring of HLA-matched and mismatched transplants.
PG  - 25-9
AB  - Thirty-six sex-mismatched transplants were studied using fluorescence in situ 
      hybridization (FISH) and polymerase chain reaction (PCR) methods. Molecular 
      cytogenetics was performed using interphase FISH with a centromeric probe for 
      chromosome Y, and PCR amplification was performed with a set of VNTR 
      microsatellite loci. In addition, reverse transcriptase-PCR (RT-PCR) for BCR-ABL 
      fusion was used to investigate cases of Philadelphia chromosome (Ph)-positive 
      chronic myeloid leukemia (CML) and acute lymphoblastic leukemia (ALL). Our 
      integrated approach of post-transplant monitoring was helpful in documenting 
      successful transplants and in controlling the size of Ph-positive clones in CML. 
      A striking overlap was found between results from FISH analysis and PCR for 
      polymorphic loci.
FAU - Crescenzi, B
AU  - Crescenzi B
AD  - Hematology and Bone Marrow Transplantation Unit, University of Perugia, Perugia, 
      Italy.
FAU - Fizzotti, M
AU  - Fizzotti M
FAU - Piattoni, S
AU  - Piattoni S
FAU - La Starza, R
AU  - La Starza R
FAU - Matteucci, C
AU  - Matteucci C
FAU - Carotti, A
AU  - Carotti A
FAU - Aversa, F
AU  - Aversa F
FAU - Martelli, M F
AU  - Martelli MF
FAU - Mecucci, C
AU  - Mecucci C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (HLA Antigens)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Bone Marrow Transplantation
MH  - Child
MH  - Female
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - HLA Antigens
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Histocompatibility
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics/therapy
MH  - Male
MH  - Middle Aged
MH  - *Minisatellite Repeats
MH  - Polymorphism, Genetic
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics/therapy
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Y Chromosome
EDAT- 2000/07/29 11:00
MHDA- 2000/08/12 11:00
CRDT- 2000/07/29 11:00
PHST- 2000/07/29 11:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 2000/07/29 11:00 [entrez]
AID - S0165-4608(99)00245-9 [pii]
AID - 10.1016/s0165-4608(99)00245-9 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2000 Jul 1;120(1):25-9. doi: 
      10.1016/s0165-4608(99)00245-9.