PMID- 10915573
OWN - NLM
STAT- MEDLINE
DCOM- 20000831
LR  - 20220310
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 164
IP  - 2
DP  - 2000 Aug
TI  - Effects of endogenous neurotrophins on sympathetic sprouting in the dorsal root 
      ganglia and allodynia following spinal nerve injury.
PG  - 344-50
AB  - Peripheral nerve injury is often complicated by a chronic pain syndrome that is 
      difficult to treat. In animal models of peripheral nerve injury, sympathetic 
      nerve terminals in the dorsal root ganglia (DRG) sprout to form baskets around 
      large diameter neurons, an anatomical change that has been implicated in the 
      induction of neuropathic pain. In the present study, we have investigated whether 
      neurotrophins derived from peripheral sources play any roles in sympathetic 
      sprouting and neuropathic pain in a rat model of peripheral nerve injury. After 
      transection of the left lumbar (L) 5 spinal nerve, antisera specific to 
      neurotrophins were injected intraperitoneally twice a week for 2 weeks. The foot 
      withdrawal response to von Frey hairs was examined on days 1, 3, 7, 10, and 14 
      postlesion. After completion of behavioral tests, sympathetic sprouting in DRG 
      was examined by tyrosine hydroxylase (TH) immunohistochemistry. The number of 
      TH-immunoreactive (ir) fibers and baskets around large neurons within the 
      lesioned DRG was dramatically increased in the rats treated with control normal 
      sheep serum. Antisera specific to nerve growth factor (NGF), neurotrophin-3 
      (NT3), and brain-derived neurotrophic factor (BDNF) significantly reduced the 
      sympathetic sprouting and the formation of baskets. L5 spinal nerve lesion 
      induced a significant increase in foot withdrawal responses to von Frey hair 
      stimuli, which was attenuated by treatment of antisera to neurotrophins with a 
      different time sequential. The effect of BDNF antiserum occurred earlier and 
      lasted longer than those of NGF and NT3 antisera. These results implicate that 
      peripherally derived neurotrophins are involved in the induction of sympathetic 
      sprouting and neuropathic pain following peripheral nerve injury.
CI  - Copyright 2000 Academic Press.
FAU - Deng, Y S
AU  - Deng YS
AD  - Department of Human Physiology, Flinders University of South Australia, Adelaide, 
      5001, Australia.
FAU - Zhong, J H
AU  - Zhong JH
FAU - Zhou, X F
AU  - Zhou XF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Immune Sera)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/antagonists & inhibitors/metabolism
MH  - Ganglia, Spinal/drug effects/metabolism/*pathology
MH  - Hyperalgesia/*drug therapy/physiopathology
MH  - Immune Sera/administration & dosage
MH  - Immunohistochemistry
MH  - Male
MH  - Nerve Growth Factor/antagonists & inhibitors/metabolism
MH  - Nerve Growth Factors/antagonists & inhibitors/*metabolism
MH  - Nerve Regeneration/drug effects/*physiology
MH  - Neurons/cytology/drug effects/metabolism
MH  - Neurotrophin 3/antagonists & inhibitors/metabolism
MH  - Pain/*physiopathology
MH  - Physical Stimulation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Nerves/drug effects/*injuries/physiopathology
MH  - Sympathetic Nervous System/physiopathology
MH  - Tyrosine 3-Monooxygenase/metabolism
EDAT- 2000/08/01 11:00
MHDA- 2000/09/02 11:01
CRDT- 2000/08/01 11:00
PHST- 2000/08/01 11:00 [pubmed]
PHST- 2000/09/02 11:01 [medline]
PHST- 2000/08/01 11:00 [entrez]
AID - S0014-4886(00)97432-6 [pii]
AID - 10.1006/exnr.2000.7432 [doi]
PST - ppublish
SO  - Exp Neurol. 2000 Aug;164(2):344-50. doi: 10.1006/exnr.2000.7432.