PMID- 10915603
OWN - NLM
STAT- MEDLINE
DCOM- 20000906
LR  - 20071114
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 273
IP  - 2
DP  - 2000 Aug 1
TI  - Cloning of an infectious milk-borne mouse mammary tumor virus (MMTV) DNA from a 
      mammary tumor that developed in an endogenous MMTV-free wild mouse.
PG  - 325-32
AB  - Molecular characterization of infectious mouse mammary tumor viruses (MMTVs) has 
      been hampered due to the problem of cloning a full-length exogenous virus into a 
      plasmid. The present report describes our strategy for obtaining a full-length 
      clone of an exogenous MMTV from a mouse mammary tumor that arose spontaneously in 
      a wild Chinese mouse free of endogenous MMTV and shows that the cloned virus 
      (JYG-MMTV) is expressed in rat RBA cells. Four-week-old C58/J x CBA/CaJ female 
      mice, free of both endogenous and exogenous MMTVs, were injected with 
      virus-secreting RBA cells. The progeny of these mice were bred, and their 
      offspring were tested for the presence of MMTV. These third-generation mice were 
      found to actively produce MMTV that was shed in their milk and transmitted to 
      their offspring. The virus was detected not only in the mammary glands of these 
      young mice, but also in their spleens and bone marrow. These results suggest that 
      our plasmid-cloned exogenous JYG-MMTV is infectious. This virus can now be used 
      effectively in manipulating the various genes of JYG-MMTV and other MMTV strains 
      to understand their structure/function relationships.
CI  - Copyright 2000 Academic Press.
FAU - Xu, L
AU  - Xu L
AD  - Institute of Molecular Medicine and Genetics, Medical College of Georgia, 
      Augusta, Georgia 30912, USA.
FAU - Tay, C H
AU  - Tay CH
FAU - Huber, B T
AU  - Huber BT
FAU - Sarkar, N H
AU  - Sarkar NH
LA  - eng
GR  - CA45156/CA/NCI NIH HHS/United States
GR  - R37AI14910/AI/NIAID NIH HHS/United States
GR  - T32AI07389/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Cloning, Molecular
MH  - DNA, Viral/*chemistry
MH  - Female
MH  - Mammary Neoplasms, Animal/genetics/*virology
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Milk/virology
MH  - Proviruses/genetics/pathogenicity
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transfection
EDAT- 2000/08/01 11:00
MHDA- 2000/09/09 11:01
CRDT- 2000/08/01 11:00
PHST- 2000/08/01 11:00 [pubmed]
PHST- 2000/09/09 11:01 [medline]
PHST- 2000/08/01 11:00 [entrez]
AID - S0042-6822(00)90437-9 [pii]
AID - 10.1006/viro.2000.0437 [doi]
PST - ppublish
SO  - Virology. 2000 Aug 1;273(2):325-32. doi: 10.1006/viro.2000.0437.