PMID- 10916091
OWN - NLM
STAT- MEDLINE
DCOM- 20000907
LR  - 20221207
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 58
IP  - 2
DP  - 2000 Aug
TI  - Possible relationship of monocyte chemoattractant protein-1 with diabetic 
      nephropathy.
PG  - 684-90
AB  - BACKGROUND: Monocyte chemoattractant protein-1 (MCP-1) is a specific chemokine to 
      recruit and activate monocytes from the circulation to inflammatory site. In 
      diabetic nephropathy, similar to other glomerulonephropathies, infiltration and 
      activation of monocytes/macrophages in glomerulus have been implicated in the 
      development of glomerular injury. The aim of the present study was to examine a 
      possible relationship of MCP-1 with diabetic nephropathy and to investigate the 
      role of glycated albumin (Gly-Alb) as well as high concentration of glucose (HG) 
      on MCP-1 production by cultured human mesangial cells. METHODS: MCP-1 in serum or 
      urine and urinary albumin (Alb) as well as several clinical parameters such as 
      plasma glucose, serum Gly-Alb, and hemoglobin A1c (HbA1c) were measured after 
      overnight fasting in 16 control subjects and 54 diabetic patients. The 
      relationships between the levels of urinary Alb and urinary or serum MCP-1 and 
      also between the values of respective clinical parameter and urinary MCP-1 levels 
      were analyzed. Next, using cultured human mesangial cells, we investigated the 
      role of Gly-Alb and/or HG on the gene and protein expression of MCP-1. RESULTS: 
      Urinary levels (ng/g creatinine), but not serum levels, of MCP-1 increased in 
      accordance with the extent of albuminuria. In all subjects, there were 
      significant correlations between the urinary levels of Alb and MCP-1 (r = 0.746, 
      P < 0.0001) and between the levels of serum Gly-Alb and urinary MCP-1 (r = 0.475, 
      P < 0.0001). In cultured human mesangial cells, the gene and protein expression 
      of MCP-1 was dose and time dependently up-regulated by Gly-Alb. HG slightly but 
      significantly stimulated MCP-1 expression. The combination of Gly-Alb and HG 
      showed the greatest stimulation in more than an additive manner on MCP-1 
      production. CONCLUSIONS: This study suggests that facilitated MCP-1 production by 
      mesangial cells in diabetic milieu contributes to the initiation and progression 
      of diabetic nephropathy.
FAU - Banba, N
AU  - Banba N
AD  - Department of Endocrinology and Metabolism, Dokkyo University School of Medicine, 
      Tochigi, Japan.
FAU - Nakamura, T
AU  - Nakamura T
FAU - Matsumura, M
AU  - Matsumura M
FAU - Kuroda, H
AU  - Kuroda H
FAU - Hattori, Y
AU  - Hattori Y
FAU - Kasai, K
AU  - Kasai K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Blood Glucose)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (Serum Albumin)
RN  - 0 (Glycated Serum Albumin)
SB  - IM
MH  - Adult
MH  - Albuminuria/blood/immunology/urine
MH  - Blood Glucose
MH  - Cells, Cultured
MH  - Chemokine CCL2/*blood/metabolism/*urine
MH  - Diabetes Mellitus, Type 1/blood/immunology/urine
MH  - Diabetes Mellitus, Type 2/blood/immunology/urine
MH  - Diabetic Nephropathies/blood/*immunology/urine
MH  - Female
MH  - Glomerular Mesangium/cytology
MH  - Glycation End Products, Advanced
MH  - Humans
MH  - Hyperglycemia/blood/immunology/urine
MH  - Male
MH  - Middle Aged
MH  - *Serum Albumin
MH  - Glycated Serum Albumin
EDAT- 2000/08/01 11:00
MHDA- 2000/09/09 11:01
CRDT- 2000/08/01 11:00
PHST- 2000/08/01 11:00 [pubmed]
PHST- 2000/09/09 11:01 [medline]
PHST- 2000/08/01 11:00 [entrez]
AID - S0085-2538(15)47148-0 [pii]
AID - 10.1046/j.1523-1755.2000.00214.x [doi]
PST - ppublish
SO  - Kidney Int. 2000 Aug;58(2):684-90. doi: 10.1046/j.1523-1755.2000.00214.x.