PMID- 10917138
OWN - NLM
STAT- MEDLINE
DCOM- 20001102
LR  - 20071115
IS  - 0361-090X (Print)
IS  - 0361-090X (Linking)
VI  - 24
IP  - 2
DP  - 2000
TI  - Fluorescence in situ hybridization evaluation of minimal residual disease on 
      stem-cell harvests.
PG  - 169-72
AB  - The usefulness of fluorescence in situ hybridization (FISH) analysis to detect 
      minimal residual disease (MRD) in autologous bone marrow and peripheral blood 
      stem-cell harvests has been tested in three patients with hematologic 
      malignancies. Conventional cytogenetics and FISH were used to characterize the 
      leukemic clones identifying the specific chromosomal abnormalities (monosomy 7 in 
      a myelodysplastic patient and trisomy 8 in two acute myeloid leukemic patients). 
      Such analysis was useful to monitor the MRD persistent after treating these 
      patients with intensive chemotherapy. The myelodysplastic patient underwent eight 
      peripheral blood-stem cell harvests in which FISH detected the persistence of 
      monosomy 7 cells, precluding their use for autologous transplantation. This 
      patient relapsed and died. In two acute myeloid leukemia patients who underwent 
      an autologous marrow harvest, FISH did not show a significant proportion of 
      trisomy 8 cells. Nevertheless, autologous transplantation was not performed, 
      owing to an insufficient CD34 cell content in the harvests. One of these patients 
      relapsed with the reappearance of trisomy 8 and died. The other patient, on the 
      contrary, is alive in complete remission 3 years after the bone marrow harvest. 
      The usefulness and applicability of MRD quantification in stem-cell harvests is 
      discussed on the basis of the sensitivity of the methodology applied.
FAU - Diez-Martin, J L
AU  - Diez-Martin JL
AD  - Department of Haematology, Clinica Puerta de Hierro, Spain.
FAU - Buno, I
AU  - Buno I
FAU - Llamas, P
AU  - Llamas P
FAU - Gosalvez, J
AU  - Gosalvez J
FAU - Lopez-Fernandez, C
AU  - Lopez-Fernandez C
FAU - Polo, N
AU  - Polo N
FAU - Regidor, C
AU  - Regidor C
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cancer Detect Prev
JT  - Cancer detection and prevention
JID - 7704778
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Chromosome Aberrations
MH  - Female
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Myeloid/diagnosis/*genetics/*therapy
MH  - Leukemia, Myeloid, Acute/diagnosis/genetics/therapy
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/diagnosis/genetics/therapy
MH  - Neoplasm, Residual/*diagnosis
MH  - Tissue and Organ Harvesting
MH  - Transplantation, Autologous
EDAT- 2000/08/05 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/08/05 11:00
PHST- 2000/08/05 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/08/05 11:00 [entrez]
PST - ppublish
SO  - Cancer Detect Prev. 2000;24(2):169-72.